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Session V: Immunotherapy I

Bcr/abl+ autologous dendritic cells for vaccination in chronic myeloid leukemia

Abstract

In chronic myeloid leukemia (CML) ex vivo generated DC are characterized by constitutive expression of bcr/abl and possibly other yet undefined leukemia-associated antigens, since these DC share a common progeny with leukemic cells. Induction of anti-leukemic T cell responses has been described in vitro. For a phase I vaccination study, autologous bcr/abl+ DC are generated under GMP conditions mainly from monocyte precursors in chronic phase CML patients. Lin, CD80+, CD86+, CD83+, DR+ DC could be generated in sufficient numbers for s.c. vaccination with 1 × 106–5 × 107 DC. Using monocyte precursors, the yield of DC per seeded PBMC was in the range of 1–6%. Furthermore, we could demonstrate in vitrothat the T cell stimulatory ability of CD34+-derived DC can be augmented by a factor 2–3 by retroviral transduction with a gene coding for interleukin-7. DC-based vaccination strategies are a promising clinical approach, particularly as post-remission immunotherapy in the setting of autologous stem cell transplantation. Bone Marrow Transplantation (2000) 25 , Suppl. 2, S46–S49.

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Westermann, J., Kopp, J., Körner, I. et al. Bcr/abl+ autologous dendritic cells for vaccination in chronic myeloid leukemia. Bone Marrow Transplant 25, S46–S49 (2000). https://doi.org/10.1038/sj.bmt.1702354

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  • DOI: https://doi.org/10.1038/sj.bmt.1702354

Keywords

  • dendritic cells
  • CML
  • vaccination
  • interleukin-7

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