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Progenitor Cell Mobilisation

Lenograstim-mobilized peripheral blood progenitor cells in volunteer donors: an open label randomized split dose escalating study

Bone Marrow Transplantation volume 25, pages 371–376 (2000)Cite this article

Abstract

Mobilization of peripheral blood cell progenitor cells was investigated in 36 healthy sibling donors using three different split doses of glycosylated rhG-CSF (lenograstim). The donors were randomized into three groups: group 1 was given lenograstim at 8, group 2 at 11 and group 3 at 15 μg/kg/day in two split doses, subcutaneously for 4 and 5 days, respectively. Leukapheresis was performed on day 4 or 5 depending on the WBC and CD34+ cell count. We were able to demonstrate that there was a significant correlation between circulating CD34+cells on the day of harvest and CD34+ cells in the apheresis products in all three groups. The number of CD34+ cells pre-apheresis was inversely correlated with age in group 1 and group 2. However, in group 3, the number of CD34+ cells pre-apheresis did not correlate with age. There was also a difference between the number of progenitor cells mobilized in the three dose groups regarding the time of harvest. Apheresis was performed in groups 1 and 2 on day 5 of mobilization in order to obtain a sufficient number of stem cells for allogeneic transplantation. In contrast, with the split dose of 15 μg/kg/day, harvest could be routinely performed on day 4 of stimulation. We conclude that lenograstim given twice a day at doses of 8, 11 and 15 μg/kg/day provided different CD34+ cell yields in normal donors, in particular, with regard to the time of harvest. The number of CD34+cells pre-apheresis was not correlated with age in the group of donors mobilized with a split dose of 15 μg/kg/day, indicating that this dosage might also be suitable for older donors. Bone Marrow Transplantation (2000) 25, 371–376.

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  1. Clinic of Bone Marrow Transplantation and Haematology/Oncology and European Institute for Research and Development of Transplant Strategies, Idar-Oberstein, Germany

    N Basara, B Schmetzer, IW Blau, M Bischoff, S Günzelmann, D Kirsten & AA Fauser

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  1. N Basara
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  2. B Schmetzer
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Basara, N., Schmetzer, B., Blau, I. et al. Lenograstim-mobilized peripheral blood progenitor cells in volunteer donors: an open label randomized split dose escalating study. Bone Marrow Transplant 25, 371–376 (2000). https://doi.org/10.1038/sj.bmt.1702168

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