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Graft Versus Host Disease

Decreased graft-versus-host disease after haplotype mismatched bone marrow allografts in miniature swine following interleukin-2 treatment

Bone Marrow Transplantation volume 25, pages 47–52 (2000)Cite this article

Abstract

Graft-versus-host disease (GVHD) is an important complication of bone marrow transplantation after transplants between HLA-mismatched donor/recipient pairs. In mice, giving IL-2 post transplant decreases GVHD in this setting. We studied high-dose IL-2 therapy in pigs. Transplants were carried out after conditioning with fractionated total body radiation and cyclophosphamide. Fourteen pigs received a fully mismatched bone marrow transplant (six with IL-2; eight without IL-2), and six received a single haplotype class II mismatched transplant (three with IL-2; three without IL-2). GVHD was evaluated by skin histology. All fully mismatched recipients had severe GVHD (grade 2–3) and died within 13 to 51 days whether or not they received IL-2. Pigs receiving a one haplotype class II mismatched transplant without IL-2 developed severe skin GVHD lasting for 8–45 days; all died within 57 days. Similar pigs receiving IL-2 post transplant had no or only mild skin GVHD for less than 15 days; two are long-term survivors. Bone Marrow Transplantation (2000) 25, 47–52.

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Acknowledgements

We would like to thank Drs Gary Haller and Yasushi Fuchimoto for their helpful reviews of the manuscript, and Lisa A Bernardo for help in manuscript preparation. This work was supported by a National Institutes of Health Grant No. 1 RO1 CA61537 and by a Sponsored Research Agreement between the Massachusetts General Hospital and BioTransplant, Inc. T Kozlowski is a recipient of a Sandoz Fellowship in Transplantation Award sponsored by the American Society of Transplant Physicians.

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Author notes

  1. T Sablinski & H Kitamura

    Present address: Novartis, East Hanover, NJ, USA

Authors and Affiliations

  1. Transplantation Biology Research Center, Massachusetts General Hospital/Harvard Medical School, Boston, Massachusetts, USA

    T Kozlowski, T Sablinski, M Basker, M Sykes, DKC Cooper & DH Sachs

  2. Department of Pathology, Massachusetts General Hospital/Harvard Medical School, Boston, Massachusetts, USA

    H Kitamura

  3. Department of Medicine, (Hematology/Oncology Unit), Massachusetts General Hospital/Harvard Medical School, Boston, Massachusetts, USA

    TR Spitzer

  4. Department of Infectious Diseases, Massachusetts General Hospital/Harvard Medical School, Boston, Massachusetts, USA

    J Fishman

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  1. T Kozlowski
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Kozlowski, T., Sablinski, T., Basker, M. et al. Decreased graft-versus-host disease after haplotype mismatched bone marrow allografts in miniature swine following interleukin-2 treatment. Bone Marrow Transplant 25, 47–52 (2000). https://doi.org/10.1038/sj.bmt.1702083

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