Progenitor Cell Mobilisation

Characterization of peripheral blood progenitor cells mobilized by nartograstim (N-terminal replaced granulocyte colony-stimulating factor) in normal volunteers

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Abstract

We performed an optimal dose-finding study of nartograstim, N-terminal amino acids-replaced rhG-CSF, for mobilization of PBPC in normal volunteers. Nartograstim was injected subcutaneously for 5 days (days 1–5) at a dose of 1 (n = 3), 2 (n = 3), 4 (n = 6) or 8 μg/kg/day (n = 6), and blood samples were obtained by venipuncture on days 1 (pre-treatment), 4, 5 and 6. Nartograstim was well tolerated up to 8 μg/kg/day. Many kinds of PBPC, such as various hematopoietic progenitors in clonal culture, long-term culture-initiating cells (LTC-IC), and CD34+ cells and their primitive subsets (CD33, HLA-DR, CD38) were mobilized in a dose-related manner. At 8 μg/kg/day, the peak number of CD34+ cells and granulocyte–macrophage colony-forming unit (CFU-GM) reached 82.8 × 103/ml and 16.7 × 103/ml, respectively; LTC-ICMix, which have the capability to produce mixed colony-forming units (CFU-Mix) for over 5 weeks on stromal cells, were detected after the administration of nartograstim. There is a significant relationship between the number of mobilized CD34+ cells and the number of various progenitor cells including LTC-IC. These results indicate that a 5-day administration of nartograstim at 8 μg/kg/day could mobilize PBPC effectively for allografting.

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Correspondence to T Nakahata.

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Maruyama, K., Tsuji, K., Tanaka, R. et al. Characterization of peripheral blood progenitor cells mobilized by nartograstim (N-terminal replaced granulocyte colony-stimulating factor) in normal volunteers. Bone Marrow Transplant 22, 313–320 (1998) doi:10.1038/sj.bmt.1701333

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Keywords

  • PBPC
  • mobilization
  • G-CSF
  • CD34+ subsets
  • colony-forming cell
  • long-term culture-initiating cell (LTC-IC)

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