Efficacy and kinetics of bone marrow processing and enrichment of haematopoietic progenitor cells (HPC) by a large-volume apheresis procedure

Abstract

We investigated the efficacy of bone marrow (BM) processing by an automated large-volume apheresis procedure (6 × original BM volume) in 10 paediatric and adult patients undergoing BM harvesting before myeloablative therapy. Volume-dependent kinetics during apheresis were analyzed by sequential collection of processed cells into a six-fold collection bag system with consecutive analysis of the single bags. BM processing resulted in an 83.3% (±21) recovery of mononuclear cells (MNC), a 97.9% (±1.1) reduction of erythrocytes (RBC) and a 87.7% (±2.9) volume reduction. To determine volume-dependent kinetics of haematopoietic progenitor cell (HPC) enrichment during apheresis, leukocytes (WBC), mononuclear cells (MNC), CD34⁺ cells and colony-forming cells (CFU-GM) were serially quantitated in subsequent collection bags. Large-volume BM processing significantly enhanced absolute yields of CD34⁺ cells (mean: 4.01 (±2.81) × 10⁶/kg bw) and CFU-GM (mean: 1.92 (±1.47) × 10⁴/kg bw) compared with the standard procedure (3 × BM volume) by 26.9% (±10.9) and 27.2% (±11.6), respectively. We concluded that large-volume apheresis for BM processing is an efficient technique significantly improving the yields of haematopoietic progenitor cells (HPC) without any relevant changes in the purity of the final product. Moreover, sequential collection and analysis of HPC represents a good model to investigate the volume-dependent kinetics and efficacy of BM processing.