Letter | Published:

Purification of Commercial Samples of Œstrone

Naturevolume 169pages967968 (1952) | Download Citation



PAPER chromatography of the p-nitrobenzene-azodimethoxyaniline (‘Fast Black Salt K’) derivatives of two commercial samples of œstrone, according to the method described by Heftmann1, revealed the presence of small traces of an impurity which appeared as a blue spot with lower RF value than the pink œstrone dye. This substance could be removed from the œstrone by means of partition chromatography on a ‘Celite’–sodium hydroxide column, following the method of Swyer and Braunsberg2. Paper chromatograms, run before and after purification by our technique, showed that the impurity was completely removed by this means (see photograph).

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  1. 1

    Heftmann, E., Science, 111, 571 (1950); J. Amer. Chem. Soc., 73, 851 (1951).

  2. 2

    Swyer, G. I. M., and Braunsberg, H., J. Endocrin., 7, lx (1951).

  3. 3

    Mentioned by Carol, J., J. Amer. Pharm. Assoc., Sci. Ed., 39, 425 (1951).

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  1. University College Hospital Medical School, London, W.C.1



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