Letter | Published:

Isolation of Nitrifying Organisms and Oxygen Supply

Naturevolume 169pages11061107 (1952) | Download Citation



IN a communication, “Isolation of the Nitrifying Organisms from Soil”1, Dr. Howard Lees stated that he believed that he obtained a pure culture of Nitrosomonas with his percolator method. The only one who, so far, has got a pure culture of Nitrosomonas without any microbiological technique has been Bonazzi. Lees ascribes the success of this technique to the good aeration the percolator method provides to the culture. Bonazzi also aerated his cultures, but he hesitates to ascribe his success only to aeration. This experience led Lees to the conclusion that the well-known difficulties encountered in getting pure cultures of the nitrifiers were due to a lack of oxygen supply. This view he tries to justify by referring to correct figures on oxygen consumption of nitrifying cultures and diffusion-rate of oxygen taken respectively from Stephenson and Höber.

Access optionsAccess options

Rent or Buy article

Get time limited or full article access on ReadCube.


All prices are NET prices.


  1. 1

    Lees, H., Nature, 167, 355 (1951).

  2. 2

    Kingma Boltjes, T. Y., Arch. Mikrob., 6, 79 (1935). Hes, J. W., Rec. Tr. Bot. néerlandais, 34, 234 (1937).

  3. 3

    Hanks, J. H., and Weintraub, R. L., J. Bact., 32, 653 (1936). Meiklejohn, J., J. Gen. Microb., 4, 185 (1950).

Download references

Author information


  1. Laboratory of Microbiology, University of Amsterdam



  1. Search for T. Y. KINGMA BOLTJES in:

About this article

Publication history

Issue Date




By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.