Letter | Published:

Chromatographic Detection of Acids in Cultures of Aspergillus niger on Various Substrates

Naturevolume 169pages10631064 (1952) | Download Citation



UNDER this title, Walker, Hall and Hopton1 have described the formation of pyruvic and α-keto-glutaric acids from a glucose–arsenite medium. Further evidence for the close relationship of the metabolism of Aspergillus niger to the tricarboxylic acid cycle is now reported with the simultaneous identification of oxalic, gluconic, citric, malic, glycollic, succinic, pyruvic and fumaric acids. These products were separated for analysis on filter paper as free acids2 or as the 2:4 dinitrophenylhydrazones3. Approximate quantitative determinations were made by comparing the unknown spots with a range of standard acids of known concentration. Citrate and oxalate were also examined by more accurate methods4,5. All metabolic solutions were inoculated with a spore suspension of A. niger, which rapidly formed an actively growing mycelium.

Access optionsAccess options

Rent or Buy article

Get time limited or full article access on ReadCube.


All prices are NET prices.


  1. 1

    Walker, T. K., Hall, A. N., and Hopton, J. W., Nature, 168, 1042 (1951).

  2. 2

    Lugg, J. W. H., and Overell, B. T., Nature, 160, 87 (1947); Aust. J. Sci. Research, A, 1, 98 (1948).

  3. 3

    Cavallini, D., Frontali, N., and Toschi, G., Nature, 163, 568; 164, 792 (1949).

  4. 4

    Taussky, H. H., and Shorr, E., J. Biol. Chem., 169, 103 (1947).

  5. 5

    Halliwell, G., Anal. Chem., 22, 1184 (1950).

  6. 6

    Currie, J. N., J. Biol. Chem., 31, 15 (1917).

  7. 7

    Challenger, F., Subramaniam, V., and Walker, T. K., J. Chem. Soc., 200, 3044 (1927).

  8. 8

    Lynen, F., and Lynen, F., Ann., 560, 149 (1948).

  9. 9

    Deniges, G., Ann. chim. Phys., 18, 178 (1909). Neuberg, C., Biochem. Z., 24, 436 (1910).

  10. 10

    Sakaguchi, K., and Baba, S., J. Agric. Chem. Soc. Japan, 18, 1127 (1942).

Download references

Author information


  1. Courtauld Institute, Middlesex Hospital, London, W.1



  1. Search for G. HALLIWELL in:

About this article

Publication history

Issue Date



Further reading


By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.