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Chromatographic Detection of Acids in Cultures of Aspergillus niger on Various Substrates

Nature volume 169, pages 10631064 (21 June 1952) | Download Citation

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Abstract

UNDER this title, Walker, Hall and Hopton1 have described the formation of pyruvic and α-keto-glutaric acids from a glucose–arsenite medium. Further evidence for the close relationship of the metabolism of Aspergillus niger to the tricarboxylic acid cycle is now reported with the simultaneous identification of oxalic, gluconic, citric, malic, glycollic, succinic, pyruvic and fumaric acids. These products were separated for analysis on filter paper as free acids2 or as the 2:4 dinitrophenylhydrazones3. Approximate quantitative determinations were made by comparing the unknown spots with a range of standard acids of known concentration. Citrate and oxalate were also examined by more accurate methods4,5. All metabolic solutions were inoculated with a spore suspension of A. niger, which rapidly formed an actively growing mycelium.

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Affiliations

  1. Courtauld Institute, Middlesex Hospital, London, W.1. Jan. 16.

    • G. HALLIWELL

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https://doi.org/10.1038/1691063a0

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