Abstract
THE biological activity of growth hormone1 is found to be greatly reduced following treatment with acetic anhydride, which is known to acetylate protein amino groups specifically2. The relative importance of free α- and ɛ-amino groups has not hitherto been investigated. Purified growth hormone has now been acetylated according to the conditions of Li and Evans1, but with smaller amounts of acetic anhydride so as to avoid complete inactivation.
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References
Li, C. H., and Evans, H. M., “Recent Progress in Hormone Research” (edit. G. Pincus), chap. 1, Vol. 3 (New York: Academic Press, Inc., 1948).
Fraenkel-Conrat, J., and Fraenkel-Conrat, H., Biochim. et Biophys. Acta, 5, 89 (1950).
Sanger, F., Biochem. J., 39, 507 (1945).
Li, C. H., and Porter, R. R. (unpublished experiment).
Cotes, P. M., Reid, E., and Young, F. G., J. Endocrinol., 6, xiv (1949).
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REID, E. Relative Importance of Free α- and ɛ-Amino Groups for the Biological Activity of the Growth Hormone. Nature 168, 955 (1951). https://doi.org/10.1038/168955a0
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DOI: https://doi.org/10.1038/168955a0
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