Electric Shock and the Regeneration of the Corneal Epithelium of Rabbits


IT was considered of interest to discover the effect of electric shock on epithelial regeneration, since there are indications that mitotic activity is profoundly affected in shock conditions1. In order to obtain quantitative information, artificially produced lesions of the corneal epithelium of rabbits were regarded as a suitable object. With the aid of Nižetić's corneal trepan and a small lancet, practically identical lesions were made in each cornea. Each of them had a diameter of 4.5 mm. and for convenience was dyed with uranin (1 per cent); thus it was easy to measure two diameters at right angles to each other. There were two groups, each consisting of four animals, and both eyes of each animal were operated on. One group of animals was stressed by electrical injury three times a day during observation, by applying alternating current of 50 c./s. and 45 V. for one second. Convulsions lasted for nearly 60 sec., unconsciousness up to 180 sec. Except during the convulsions, the sensitiveness of the cornea was normal. The other group served as a control. To obtain information as to the speed of regeneration, the extent of the two diameters of each lesion was measured with a circle every three hours during the day-time.


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    Bullough, W. S., and Green, H. N., Nature, 164, 795 (1949).

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HOLZBAUER, M., RIGLER, R. Electric Shock and the Regeneration of the Corneal Epithelium of Rabbits. Nature 168, 919 (1951). https://doi.org/10.1038/168919a0

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