THE technique described was developed for the purpose of studying the action of chemotherapeutic agents, given in divided doses by the yolk-sac route, on the proliferation of vaccinia virus in embryonated eggs.
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COLLIER, L. A Method for Administering Chemotherapeutic Agents to Chick Embryos. Nature 168, 832 (1951). https://doi.org/10.1038/168832a0