Determination and Occurrence of Fluorescent Substances in the Neutral Fraction of Urinary Extracts

Abstract

IT has been shown that the natural phenolic œstrogens produce fluorescence on being heated with phosphoric acid1,2. When applying this method to urinary extracts, it was found that a non-specific fluorescence obscures the readings for the œstrogens, especially in the ‘strong phenolic fraction’—the œstriol fraction. On attempting to purify the œstrogenic fractions of the non-specific fluorescence, we observed that a great part of it was non-phenolic, but was due to a ‘neutral’ substance or substances, which were carried over in the partition procedure then used (a modified Bachmann–Pettit method2) to the œstrogenic fraction.

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References

  1. 1

    Finkelstein, M., Hestrin, S., and Koch, W., Proc. Soc. Exp. Biol. and Med., 64, 64 (1947).

  2. 2

    Finkelstein, M., Proc. Soc. Exp. Biol. and Med., 69, 181 (1948).

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ZONDEK, B., FINKELSTEIN, M. Determination and Occurrence of Fluorescent Substances in the Neutral Fraction of Urinary Extracts. Nature 168, 831–832 (1951). https://doi.org/10.1038/168831a0

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