Abstract
THE fluorometric analysis of œstrogens in urine using phosphoric acid for the development of the fluorescence has already been described1,2. A difficulty was observed in applying this method to urines in which the œstrogen titre was low, for example, to non-pregnancy urines, since the values obtained with the fluorometric method for normal male and female urines were higher than those yielded by the bioassay (Allen–Doisy test). In addition, a ‘quenching’ effect has been observed. These findings suggested that another substance or substances were augmenting the fluorescence produced by the œstrogens. The ‘non-specific fluorescence’ has also been observed by Engel3,4, who prepared his urinary extracts by the countercurrent partition method.
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References
Finkelstein, M., Hestrin, S., and Koch, W., Proc. Soc. Exp. Biol. and Med., 64, 64 (1947).
Finkelstein, M., Proc. Soc. Exp. Biol. and Med., 69, 181 (1948).
Engel, L. L., “Recent Progress in Hormone Research”, 5 (Academic Press, New York, 1950).
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Zondek, B., Klin. Wchschr., II, 485 (1928); “Hormones of the Ovary and the Pituitary Anterior Lobe”, 84 (1st edit., Berlin, 1931).
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FINKELSTEIN, M. Fluorometric Determination of Natural Œstrogens in Urine; Elimination of Fluorogenic Compounds. Nature 168, 830–831 (1951). https://doi.org/10.1038/168830b0
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DOI: https://doi.org/10.1038/168830b0
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