Fluorometric Determination of Natural Œstrogens in Urine; Elimination of Fluorogenic Compounds

Abstract

THE fluorometric analysis of œstrogens in urine using phosphoric acid for the development of the fluorescence has already been described1,2. A difficulty was observed in applying this method to urines in which the œstrogen titre was low, for example, to non-pregnancy urines, since the values obtained with the fluorometric method for normal male and female urines were higher than those yielded by the bioassay (Allen–Doisy test). In addition, a ‘quenching’ effect has been observed. These findings suggested that another substance or substances were augmenting the fluorescence produced by the œstrogens. The ‘non-specific fluorescence’ has also been observed by Engel3,4, who prepared his urinary extracts by the countercurrent partition method.

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References

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    Finkelstein, M., Hestrin, S., and Koch, W., Proc. Soc. Exp. Biol. and Med., 64, 64 (1947).

  2. 2

    Finkelstein, M., Proc. Soc. Exp. Biol. and Med., 69, 181 (1948).

  3. 3

    Engel, L. L., “Recent Progress in Hormone Research”, 5 (Academic Press, New York, 1950).

  4. 4

    Engel, L. L., Slaunwhite, W. R., Carter, P., and Nathanson, I. T., J. Biol. Chem., 185, 255 (1950).

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    Zondek, B., Klin. Wchschr., II, 485 (1928); “Hormones of the Ovary and the Pituitary Anterior Lobe”, 84 (1st edit., Berlin, 1931).

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FINKELSTEIN, M. Fluorometric Determination of Natural Œstrogens in Urine; Elimination of Fluorogenic Compounds. Nature 168, 830–831 (1951). https://doi.org/10.1038/168830b0

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