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Free Amino Groups of Collagen

Naturevolume 168pages514515 (1951) | Download Citation



USING the technique originated by Sanger1 for the determination of end-groups in proteins by reaction with dinitrofluorbenzene, no free α-amino- or imino-groups have been detected in ox hide collagen2, even when the equivalent of 2–3 gm. dinitrophenyl-collagen was placed on the silica gel columns.

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  1. 1

    Sanger, Biochem. J., 39, 507 (1945).

  2. 2

    Bowes and Kenten, Biochem. J., 43, 358 (1948).

  3. 3

    Pouradier and Venet, J. Chim. Phys., 47, 11 (1950).

  4. 4

    Bowes and Cave (unpublished work in these Laboratories).

  5. 5

    Bowes and Kenten, Biochem. J., 43, 365 (1948).

  6. 6

    Partridge and Davies, Nature, 165, 62 (1950).

  7. 7

    Adair, Partridge and Davies, Nature, 167, 605 (1951).

  8. 8

    Porter, Biochem. et Biophys. Acta, 2, 105 (1948).

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  1. British Leather Manufacturers' Research Association, Milton Park, Egham, Surrey

    • J. H. BOWES
    •  & J. A. MOSS


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