Letter | Published:

Destruction of Dinitrophenyl Amino-Acids by Tryptophane

Naturevolume 168pages390391 (1951) | Download Citation



THE only dinitrophenyl amino-acids revealed by paper chromatography1 and the use of silica columns2 during an attempt to identify and estimate the free amino groups of lysozyme by the method of Sanger2 were σ-dinitrophenyl-lysine and bis-dinitrophenyl-lysine. The recovery of the former from hydrolysed dinitrophenyl-lysozyme, using silica columns, suggested that lysozyme contains five non-terminal lysine residues; but the amount of bis-dinitrophenyl-lysine recovered was only one-third of that to be expected from one N-terminal amino-group. Similar results were obtained when lysozyme was first treated with urea3, or when the conditions of reaction with 1 : 2 : 4-fluorodinitrobenzene were varied.

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  1. 1

    Davis, H. F. (private communication).

  2. 2

    Sanger, F., Biochem. J., 39, 507 (1945).

  3. 3

    Porter, R. R., Biochim. et Biophys. Acta, 2, 105 (1948).

  4. 4

    Porter, R. R., and Sanger, F., Biochem. J., 42, 287 (1948).

  5. 5

    Lewis, J. C., Snell, N. S., Hirschmann, D. J., and Fraenkel-Conrat, H., J. Biol. Chem., 186, 23 (1950).

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  1. Low Temperature Station for Research in Biochemistry and Biophysics, University of Cambridge and Department of Scientific and Industrial Research, Downing Street, Cambridge



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