Letter | Published:

Mechanism of Absorption of Inorganic Phosphate from Blood by Tissue Cells

Naturevolume 166pages184185 (1950) | Download Citation



IN the course of a study of the phosphorus metabolism of liver, evidence was obtained suggesting that the absorption of inorganic phosphate ions from the blood is not a simple exchange of ions across the cell membrane but that it requires an energy-yielding phosphorylating mechanism. Accordingly, this hypothesis was tested by experiments in which rabbit liver left in situ was perfused with homologous plasma to which a small amount of carrier-free inorganic 32PO43− was added (2–5 µc. per litre) with or without 0.01 M sodium azide. The heparinized plasma, after addition of phosphorus-32 in saline, was divided into two equal volumes to one of which sodium azide was added; the two lots were used for the control and experimental perfusions, which were carried out on the liver of litter-mate animals.

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    Sacks, J., Cold Spring Harbor Symp., 13, 180 (1948).

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    Nickerson, W. J., and Mullins, L. J., Nature, 161, 939 (1948).

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    Spiegelman, S., Kamen, M. D., and Sussman, M., Arch. Biochem., 18, 409 (1948).

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  1. National Institute for Medical Research, The Ridgeway, Mill Hill, London, N.W.7

    • G. POPJÁK


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