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Spectroscopy of Cells: the Use of a Reflecting Microscope and Spectrographic Recording

Abstract

THIS communication describes the use of a simple microspectroscopic method which permits the recording of the ultra-violet and the visible absorption characteristics of cells or portions of cells within a period of a few seconds. The apparatus consists of a light source which emits radiation throughout the ultra-violet and the visible spectrum, a microscope with a reflecting achromatic condenser and objective of numerical aperture 0.56 (designed by Mr. A. J. Kavanagh and made by the Scientific Instrument Division of the American Optical Company), and a small quartz spectrograph (Hilger).

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MELLORS, R., KEANE, J. & STREIM, H. Spectroscopy of Cells: the Use of a Reflecting Microscope and Spectrographic Recording. Nature 166, 26–27 (1950). https://doi.org/10.1038/166026a0

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