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Spectroscopy of Cells: the Use of a Reflecting Microscope and Spectrographic Recording

Nature 166, 26–27 (1950) | Download Citation

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Abstract

THIS communication describes the use of a simple microspectroscopic method which permits the recording of the ultra-violet and the visible absorption characteristics of cells or portions of cells within a period of a few seconds. The apparatus consists of a light source which emits radiation throughout the ultra-violet and the visible spectrum, a microscope with a reflecting achromatic condenser and objective of numerical aperture 0.56 (designed by Mr. A. J. Kavanagh and made by the Scientific Instrument Division of the American Optical Company), and a small quartz spectrograph (Hilger).

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References

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      Burch, C. R., Proc. Phys. Soc. Lond., 59, 41 (1947).

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      Linderstrøm-Lang, K., “Distribution of Enzymes in Tissue and Cells”, 214 (Williams and Wilkins Co., 1939).

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      1. Sloan-Kettering Institute for Cancer Research, New York, N.Y.

        • ROBERT C. MELLORS
        • , JOHN F. KEANE JUN.
        •  & HOWARD G. STREIM

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      https://doi.org/10.1038/166026a0

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