Serum Potassium by Internal Standard Flame Photometry


FLAME photometric analysis1 of cations in biological fluids and tissues is, because of its simplicity, rapidity and greater accuracy, replacing laborious chemical procedures. The vaporization of alkali metal solutions in constant amount per minute into a controlled low-temperature air–acetylene flame produces simple emission spectra, the light intensities of which, measured photo-electrically after slit isolation of the most sensitive line spectrum for the particular element (potassium red flame at 7665 A.), are practically proportional to their concentrations for low concentrations2.

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    Berry, J. W., Chappell, D. G., and Barnes, R. B., Indust. Chem. Eng., Anal. Ed., 18, 19 (1946).

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    Bernstein, R. E., S.A. J. Med. Sci., 14, 163 (1949)

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    Parks, T. D., Johnson, H. O., and Lykken, L., Anal. Chem., 20, 822 (1948).

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    Cumings, J. N., J. Clin. Path., 1, 173 (1948).

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BERNSTEIN, R. Serum Potassium by Internal Standard Flame Photometry. Nature 165, 649 (1950) doi:10.1038/165649a0

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