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Serum Tryptophane–Perchloric Acid Reaction as a Measure of Tissue Destruction


SEIBERT and her colleagues1, and other workers, found that the polysaccharide content of serum rises above the normal in certain pathological conditions, including tuberculosis and carcinoma, and it was suggested1 that this increase is associated with tissue destruction. In view of the possibility that the carbohydrate was derived from nucleic acid, Seibert et al.2 applied to serum the tryptophane – perchloric acid reaction of Cohen3, when they found a significant increase in the reaction in cases of tuberculosis and several types of carcinoma. The nature of the reacting substance is unknown; but Seibert et al. have produced evidence suggesting that it is derived from proteins. Presumably an increased reaction may also be regarded as being associated with tissue destruction.

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  1. Seibert, F. B., Seibert, M. V., Atno, A. J., and Campbell, H. W., J. Clin. Invest., 26, 90 (1947).

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  2. Seibert, F. B., Pfaff, M. L., and Seibert, M. V., Arch. Biochem., 18, 279 (1948).

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  3. Cohen, S. S., J. Biol. Chem., 156, 691 1944).

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  4. Perlmann, G. E., Glenn, W. W. L., and Kaufman, D., J. Clin. Invest., 22, 627 (1943). Chanutin, A., and Gjessing, E. C., J. Biol. Chem., 165, 421 (1946).

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KEYSER, J. Serum Tryptophane–Perchloric Acid Reaction as a Measure of Tissue Destruction. Nature 164, 889–890 (1949).

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