Skip to main content

Thank you for visiting You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Letter
  • Published:

Origin of Hyaluronidase in the Rat Testis


DURAN-REYNALS1 demonstrated that mammalian testes contained a factor—the spreading factor—which was capable of increasing the permeability of the connective tissue. This observation has been confirmed by other investigators, and the factor has been identified as the enzyme hyaluronidase. Sprunt et al.2 showed that hyaluronidase was present in testes from adult rats, but not in testes from infantile or cryptorchid rats. In their opinion, the formation of hyaluronidase depended on complete spermatogenesis.

This is a preview of subscription content, access via your institution

Access options

Buy this article

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others


  1. Duran-Reynals, F., J. Exp. Med., 50, 527 (1929); 55, 703 (1932); 58, 101 (1933).

    Google Scholar 

  2. Sprunt, D. H., Hooker, C. W., and Raper, J. S., Proc. Soc. Exp. Biol. and Med., 41, 398 (1939).

    Article  Google Scholar 

  3. Eichenberger, E., GynÅ"cologia, 121, 288 (1946).

    CAS  Google Scholar 

  4. Kurzrok, R., Leonard, S., and Conrad, H., Amer. J. Med., 1, 491 (1946).

    Article  CAS  Google Scholar 

  5. Werthessen, N. T., Berman, S., Greenberg, B. E., and Gargill, S. L., J. Urology, 54, 565 (1945).

    Article  CAS  Google Scholar 

  6. Riisfeldt, Ove, Z. Vitamin, Hormon- und Fermentforsch, (in the press).

  7. Hale, C. W., Biochem. J., 38, 368 (1944).

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations


Rights and permissions

Reprints and permissions

About this article

Cite this article

RIISFELDT, O. Origin of Hyaluronidase in the Rat Testis. Nature 163, 874–875 (1949).

Download citation

  • Issue Date:

  • DOI:

This article is cited by


By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.


Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing