Quantitative Chromatographic Analysis of Tetra-, Tri- and Di-methyl Fructoses

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IN a recent report by Gilbert and Stacey1 on the constitution of a bacterial levan, these authors state that a convenient chromatographic technique for the quantitative analysis of tetra-, tri- and di-methyl fructoses had so far eluded them. We have devised a partition chromatogram giving quantitative results in the analysis of mixtures of 1 : 3 : 4 : 6-tetramethyl, 1 : 3 : 4-trimethyl and 3 : 4-dimethyl fructoses which is applicable to the study of methylated fructosans based on these sugars. (3 : 4 : 6-Trimethyl fructose has also been examined ; but certain difficulties, not yet overcome, arise in analytical separations involving this sugar.)

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  1. 1

    Gilbert, V. E., and Stacey, M., J. Chem. Soc., 1560 (1948).

  2. 2

    Bell, D. J., J. Chem Soc., 473 (1944).

  3. 3

    Brown, F., Hirst, E. L., Hough, L., Jones, J. K. N., and Wadman, H., Nature, 161, 720 (1948).

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BELL, D., PALMER, A. Quantitative Chromatographic Analysis of Tetra-, Tri- and Di-methyl Fructoses. Nature 163, 846 (1949) doi:10.1038/163846a0

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