Letter | Published:

Production of Riboflavin and Allied Substances during the Growth of C. diphtheriæ

Nature volume 162, page 219 (07 August 1948) | Download Citation

Subjects

Abstract

WHILE studying, by paper partition chromatography1, the utilization of amino-acids during the growth of the Park Williams No. 8 strain of C. diphtheriæ, we noted a strong yellow fluorescence with ultra-violet light of culture filtrates of the organism grown on a casein hydrolysate medium prepared essentially according to the method of Mueller and Miller2. When such culture filtrates were run on chromatograms using n-butanol-acetic acid as the solvent3, two strongly fluorescent yellow spots were observed when the dried paper was placed under an ultra-violet lamp. Neither of these spots gave any colour when the paper was sprayed with 0·1 per cent (w./v.) ninhydrin in chloroform. One of them was in a position approximating to that occupied by proline and the other to that occupied by the basic amino-acids.

Access optionsAccess options

Rent or Buy article

Get time limited or full article access on ReadCube.

from$8.99

All prices are NET prices.

References

  1. 1.

    , , and , Biochem. J., 38, 224 (1944).

  2. 2.

    , and , J. Immunol., 40, 21 (1941).

  3. 3.

    , Biochem. J., 42, 238 (1948).

  4. 4.

    , and , J. Infect. Dis., 73, 106 (1943).

  5. 5.

    , Nature, 161, 349 (1948).

Download references

Author information

Affiliations

  1. Wellcome Physiological Research Laboratories, Beckenham, Kent. April 13.

    • A. J. WOIWOD
    •  & F. V. LINGGOOD

Authors

  1. Search for A. J. WOIWOD in:

  2. Search for F. V. LINGGOOD in:

About this article

Publication history

Published

DOI

https://doi.org/10.1038/162219a0

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.