Letter | Published:

Production of Riboflavin and Allied Substances during the Growth of C. diphtheriæ

Nature volume 162, page 219 (07 August 1948) | Download Citation



WHILE studying, by paper partition chromatography1, the utilization of amino-acids during the growth of the Park Williams No. 8 strain of C. diphtheriæ, we noted a strong yellow fluorescence with ultra-violet light of culture filtrates of the organism grown on a casein hydrolysate medium prepared essentially according to the method of Mueller and Miller2. When such culture filtrates were run on chromatograms using n-butanol-acetic acid as the solvent3, two strongly fluorescent yellow spots were observed when the dried paper was placed under an ultra-violet lamp. Neither of these spots gave any colour when the paper was sprayed with 0·1 per cent (w./v.) ninhydrin in chloroform. One of them was in a position approximating to that occupied by proline and the other to that occupied by the basic amino-acids.

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    , , and , Biochem. J., 38, 224 (1944).

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    , and , J. Immunol., 40, 21 (1941).

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    , Biochem. J., 42, 238 (1948).

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    , and , J. Infect. Dis., 73, 106 (1943).

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    , Nature, 161, 349 (1948).

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  1. Wellcome Physiological Research Laboratories, Beckenham, Kent. April 13.

    • A. J. WOIWOD
    •  & F. V. LINGGOOD


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