Abstract
WHILE studying, by paper partition chromatography1, the utilization of amino-acids during the growth of the Park Williams No. 8 strain of C. diphtheriæ, we noted a strong yellow fluorescence with ultra-violet light of culture filtrates of the organism grown on a casein hydrolysate medium prepared essentially according to the method of Mueller and Miller2. When such culture filtrates were run on chromatograms using n-butanol-acetic acid as the solvent3, two strongly fluorescent yellow spots were observed when the dried paper was placed under an ultra-violet lamp. Neither of these spots gave any colour when the paper was sprayed with 0·1 per cent (w./v.) ninhydrin in chloroform. One of them was in a position approximating to that occupied by proline and the other to that occupied by the basic amino-acids.
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References
Consden, R., Gordon, A. H., and Martin, A. J. P., Biochem. J., 38, 224 (1944).
Mueller, J. H., and Miller, P. A., J. Immunol., 40, 21 (1941).
Partridge, S. M., Biochem. J., 42, 238 (1948).
Wadsworth, A. B., and Crowe, M. O‘L., J. Infect. Dis., 73, 106 (1943).
Crammer, J. L., Nature, 161, 349 (1948).
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WOIWOD, A., LINGGOOD, F. Production of Riboflavin and Allied Substances during the Growth of C. diphtheriæ. Nature 162, 219 (1948). https://doi.org/10.1038/162219a0
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DOI: https://doi.org/10.1038/162219a0
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