Effect of Salt on Partition Chromatograms

Article metrics

Abstract

THE adverse effect of salt on partition chromatograms on paper has been mentioned by Consden, Gordon and Martin1, and a method for removing salt from mixtures of amino-acids and peptides has been recently described by these authors2. If such desalted solutions are then fractionated on paper chromatograms and the individual spots washed off the paper and hydrolysed with 6 N hydrochloric acid in capillary tubes, some salt is reintroduced, presumably from the glass. Electrodialytic desalting is now scarcely practicable owing to the number and very small volume of these solutions.

Access options

Rent or Buy article

Get time limited or full article access on ReadCube.

from$8.99

All prices are NET prices.

References

  1. 1

    Consden, R., Gordon, A. H., and Martin, A. J. P., Biochem. J., 38, 224 (1944).

  2. 2

    Consden, R., Gordon, A. H., and Martin, A. J. P., Biochem. J., 41, 590 (1947).

  3. 3

    Partridge, S. M., and Westall, R. G., Biochem. J., 42, 238 (1948).

Download references

Author information

Rights and permissions

Reprints and Permissions

About this article

Cite this article

CONSDEN, R., GORDON, A. Effect of Salt on Partition Chromatograms. Nature 162, 180–181 (1948) doi:10.1038/162180a0

Download citation

Further reading

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.