Abstract
THE adverse effect of salt on partition chromatograms on paper has been mentioned by Consden, Gordon and Martin1, and a method for removing salt from mixtures of amino-acids and peptides has been recently described by these authors2. If such desalted solutions are then fractionated on paper chromatograms and the individual spots washed off the paper and hydrolysed with 6 N hydrochloric acid in capillary tubes, some salt is reintroduced, presumably from the glass. Electrodialytic desalting is now scarcely practicable owing to the number and very small volume of these solutions.
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References
Consden, R., Gordon, A. H., and Martin, A. J. P., Biochem. J., 38, 224 (1944).
Consden, R., Gordon, A. H., and Martin, A. J. P., Biochem. J., 41, 590 (1947).
Partridge, S. M., and Westall, R. G., Biochem. J., 42, 238 (1948).
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CONSDEN, R., GORDON, A. Effect of Salt on Partition Chromatograms. Nature 162, 180–181 (1948). https://doi.org/10.1038/162180a0
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DOI: https://doi.org/10.1038/162180a0
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