Letter | Published:

Influence of Hydrogen Ions and Neutral Salts upon the Hydration of Interstitial Connective Tissue

Nature volume 162, pages 152153 (24 July 1948) | Download Citation



WHEN a piece of interstitial connective tissue covering the thigh muscles of a rat was placed in water adjusted to pH. 4·5, within a few hours it became opaque, white and shrunken. On the other hand, a piece treated at pH. 11·0 became translucent, grey and swollen. These changes were mutually reversible and could be repeated several times. Observations with the dark-ground microscope showed that this swelling was not due to any change in the collagen fibrils but to a widening of the spaces between them ; shrinkage Was accompanied by an obliteration of such spaces and by a condensation and crimping of the collagen bundles.

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  1. 1.

    , Nature, 159, 100 (1947).

  2. 2.

    , Arch. micr. Anat., 12, 391 (1876).

  3. 3.

    , "Physical Chemistry of Cells and Tissues", 296 (Churchill, London, 1945).

  4. 4.

    , and , Trans. Farad. Soc., 29, 132 (1933).

  5. 5.

    , and , Kolloid-Zeitschr., 31, 171 (1922).

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  1. Department of Pathology, St. Thomas‘s Hospital Medical School, London, S.E.1. March 21.

    • T. D. DAY


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