A Modified Ionophoresis Apparatus

Abstract

Consden, Gordon and Martin¹ have described an apparatus for the electrophoretic separation of molecules of low molecular weight in a solid medium. They show that rapid perfusion of the electrodes of such an apparatus is essential, in order that, even after the potential has been applied for some considerable time, there may be no appreciable change in pH. at the gel/liquid interface due to increased ionic concentration at this point. In the apparatus described in their paper, the perfusion buffer is lost, since, after bathing the electrodes, it passes into the cooling chamber beneath the gel trough, and so to waste. Two considerations led me to explore the possibilities of separating these two liquids.