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Golgi Apparatus of Living Human Testicular Cells Seen with Phase-Contrast Microscopy

Nature volume 162, pages 7677 (10 July 1948) | Download Citation



IT was pointed out previously that in the unstained living human spermatid the Golgi apparatus is sometimes discernible with bright-ground illumination, as an area slightly differing in refractive index from the remainder of the cytoplasm1. Recently, I have been able to confirm and extend these observations with the phase-contrast microscope. This instrument was of the phase-accelerating or positive type, so that dark and light regions in the image indicated higher and lower refractive indices, as a rule.

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  1. 1.

    , S. Afr. J. Sci., 37, 239 (1940).

  2. 2.

    , and , Quart. J. Mic. Sci., 78, 1 (1936).

  3. 3.

    , Quart. J. Mic. Sci., 85, 1 (1944).

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  1. Medical School, University of the Witwatersrand, Johannesburg

    • A. G. OETTLÉ


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