Decarboxylation of Oxalacetate by Blood

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Abstract

EVOLUTION of carbon dioxide from oxalacetate by washed rabbit erythrocytes at pH 7·4 has been reported previously1. Because of the solubility of carbon dioxide at that pH, this could at best be only a qualitative indication of the decarboxylation. Working in either phosphate or 2M acetate buffer, and measuring carbon dioxide formation by the standard Warburg technique, the process has been thoroughly investigated.

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References

  1. 1

    Nossal, P. M., Aust. J. Exp. Biol. Med. Sci., 26, Part 2 (in the press).

  2. 2

    Evans, E. A., Vennesland, B., and Slotin, L., J. Biol. Chem., 147, 771 (1943).

  3. 3

    Krampitz, L. O., and Werkman, C. H., Biochem. J., 35, 595 (1941).

  4. 4

    Breusch, F. L., Biochem. J., 33, 1757 (1939).

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NOSSAL, P. Decarboxylation of Oxalacetate by Blood. Nature 162, 36–37 (1948) doi:10.1038/162036a0

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