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The effect of caffeine, green tea and tyrosine on thermogenesis and energy intake

European Journal of Clinical Nutrition volume 63, pages 57–64 (2009)Cite this article

Abstract

Objectives:

To investigate the effect of three different food ingredients tyrosine, green tea extract (GTE) and caffeine on resting metabolic rate and haemodynamics, and on ad libitum energy intake (EI) and appetite.

Methods:

Twelve healthy, normal weight men (age: 23.7±2.6 years, mean±s.d.) participated in a four-way crossover, randomized, placebo-controlled, double-blind study. Treatments were administered as tablets of 500 mg GTE, 400 mg tyrosine, 50 mg caffeine, or placebo, and were separated by >3-day washout. The acute thermogenic response was measured in a ventilated hood system for 4 h following ingestion. Blood pressure, heart rate (HR), and subjective appetite sensations were assessed hourly and ad libitum EI 4 h post-dose.

Results:

Caffeine induced a thermogenic response of 6% above baseline value (72±25 kJ per 4 h, mean±s.e.) compared to placebo (P<0.0001). The thermogenic responses to GTE and tyrosine were not significantly different from placebo. Tyrosine tended to increase 4-h respiratory quotient by 1% compared to placebo (0.01±0.005, P=0.05). Ad libitum EI was not significantly different between treatments but was reduced by 8% (−403±183 kJ), 8% (−400±335 kJ) and 3% (−151±377 kJ) compared to placebo after intake of tyrosine, GTE and caffeine, respectively. No significant difference in haemodynamics was observed between treatments.

Conclusions:

Only caffeine was thermogenic in the given dose and caused no haemodynamic side effects. The sample size was probably too small to detect any appetite suppressant properties of the treatments. Further investigations are required.

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References

  • Acheson KJ, Zahorska-Markiewicz B, Pittet P, Anantharaman K, Jequier E (1980). Caffeine and coffee: their influence on metabolic rate and substrate utilization in normal weight and obese individuals. Am J Clin Nutr 33, 989–997.

    Article  CAS  Google Scholar 

  • Arciero PJ, Gardner AW, Benowitz NL, Poehlman ET (1998). Relationship of blood pressure, heart rate and behavioral mood state to norepinephrine kinetics in younger and older men following caffeine ingestion. Eur J Clin Nutr 52, 805–812.

    Article  CAS  Google Scholar 

  • Arciero PJ, Gardner AW, Calles-Escandon J, Benowitz NL, Poehlman ET (1995). Effects of caffeine ingestion on NE kinetics, fat oxidation, and energy expenditure in younger and older men. Am J Physiol 268, E1192–E1198.

    CAS  Google Scholar 

  • Astrup A, Toubro S (1993). Thermogenic, metabolic, and cardiovascular responses to ephedrine and caffeine in man. Int J Obes Relat Metab Disord 17 (Suppl 1), S41–S43.

    CAS  PubMed  Google Scholar 

  • Astrup A, Breum L, Toubro S, Hein P, Quaade F (1992). The effect and safety of an ephedrine/caffeine compound compared to ephedrine, caffeine and placebo in obese subjects on an energy restricted diet. A double blind trial. Int J Obes Relat Metab Disord 16, 269–277.

    CAS  PubMed  Google Scholar 

  • Astrup A, Toubro S, Cannon S, Hein P, Madsen J (1991). Thermogenic synergism between ephedrine and caffeine in healthy volunteers: a double-blind, placebo-controlled study. Metabolism 40, 323–329.

    Article  CAS  Google Scholar 

  • Astrup A, Toubro S, Cannon S, Hein P, Breum L, Madsen J (1990). Caffeine: a double-blind, placebo-controlled study of its thermogenic, metabolic, and cardiovascular effects in healthy volunteers. Am J Clin Nutr 51, 759–767.

    Article  CAS  Google Scholar 

  • Berube-Parent S, Pelletier C, Dore J, Tremblay A (2005). Effects of encapsulated green tea and Guarana extracts containing a mixture of epigallocatechin-3-gallate and caffeine on 24 h energy expenditure and fat oxidation in men. Br J Nutr 94, 432–436.

    Article  CAS  Google Scholar 

  • Borchardt RT, Huber JA (1975). Catechol O-methyltransferase. 5. Structure–activity relationships for inhibition by flavonoids. J Med Chem 18, 120–122.

    Article  CAS  Google Scholar 

  • Bracco D, Ferrarra JM, Arnaud MJ, Jequier E, Schutz Y (1995). Effects of caffeine on energy metabolism, heart rate, and methylxanthine metabolism in lean and obese women. Am J Physiol 269, E671–E678.

    CAS  Google Scholar 

  • Debrah K, Haigh R, Sherwin R, Murphy J, Kerr D (1995). Effect of acute and chronic caffeine use on the cerebrovascular, cardiovascular and hormonal responses to orthostasis in healthy volunteers. Clin Sci (Lond) 89, 475–480.

    Article  CAS  Google Scholar 

  • Diepvens K, Kovacs EM, Nijs IM, Vogels N, Westerterp-Plantenga MS (2005). Effect of green tea on resting energy expenditure and substrate oxidation during weight loss in overweight females. Br J Nutr 94, 1026–1034.

    Article  CAS  Google Scholar 

  • Diepvens K, Westerterp KR, Westerterp-Plantenga MS (2007). Obesity and thermogenesis related to the consumption of caffeine, ephedrine, capsaicin and green tea. Am J Physiol Regul Integr Comp Physiol 292, R77–R85.

    Article  CAS  Google Scholar 

  • Dulloo AG (1993). Ephedrine, xanthines and prostaglandin-inhibitors: actions and interactions in the stimulation of thermogenesis. Int J Obes Relat Metab Disord 17 (Suppl 1), S35–S40.

    CAS  PubMed  Google Scholar 

  • Dulloo AG (2002). Herbal simulation of ephedrine and caffeine in treatment of obesity. Int J Obes Relat Metab Disord 26, 590–592.

    Article  CAS  Google Scholar 

  • Dulloo AG, Duret C, Rohrer D, Girardier L, Mensi N, Fathi M et al. (1999). Efficacy of a green tea extract rich in catechin polyphenols and caffeine in increasing 24-h energy expenditure and fat oxidation in humans. Am J Clin Nutr 70, 1040–1045.

    Article  CAS  Google Scholar 

  • Dulloo AG, Geissler CA, Horton T, Collins A, Miller DS (1989). Normal caffeine consumption: influence on thermogenesis and daily energy expenditure in lean and postobese human volunteers. Am J Clin Nutr 49, 44–50.

    Article  CAS  Google Scholar 

  • Dulloo AG, Seydoux J, Girardier L (1994). Paraxanthine (metabolite of caffeine) mimics caffeine's interaction with sympathetic control of thermogenesis. Am J Physiol 267, E801–E804.

    CAS  PubMed  Google Scholar 

  • Dulloo AG, Seydoux J, Girardier L, Chantre P, Vandermander J (2000). Green tea and thermogenesis: interactions between catechin-polyphenols, caffeine and sympathetic activity. Int J Obes Relat Metab Disord 24, 252–258.

    Article  CAS  Google Scholar 

  • Flint A, Raben A, Blundell JE, Astrup A (2000). Reproducibility, power and validity of visual analogue scales in assessment of appetite sensations in single test meal studies. Int J Obes Relat Metab Disord 24, 38–48.

    Article  CAS  Google Scholar 

  • Hollands MA, Arch JR, Cawthorne MA (1981). A simple apparatus for comparative measurements of energy expenditure in human subjects: the thermic effect of caffeine. Am J Clin Nutr 34, 2291–2294.

    Article  CAS  Google Scholar 

  • Hull KM, Maher TJ (1990). -tyrosine potentiates the anorexia induced by mixed-acting sympathomimetic drugs in hyperphagic rats. J Pharmacol Exp Ther 255, 403–409.

    CAS  PubMed  Google Scholar 

  • Hull KM, Maher TJ (1991). -tyrosine fails to potentiate several peripheral actions of the sympathomimetics. Pharmacol Biochem Behav 39, 755–759.

    Article  CAS  Google Scholar 

  • Hull KM, Maher TJ (1992). Effects of L-tyrosine on mixed-acting sympathomimetic-induced pressor actions. Pharmacol Biochem Behav 43, 1047–1052.

    Article  CAS  Google Scholar 

  • James JE (1994). Chronic effects of habitual caffeine consumption on laboratory and ambulatory blood pressure levels. J Cardiovasc Risk 1, 159–164.

    Article  CAS  Google Scholar 

  • Jessen A, Buemann B, Toubro S, Skovgaard IM, Astrup A (2005). The appetite-suppressant effect of nicotine is enhanced by caffeine. Diabetes Obes Metab 7, 327–333.

    Article  CAS  Google Scholar 

  • Jessen AB, Toubro S, Astrup A (2003). Effect of chewing gum containing nicotine and caffeine on energy expenditure and substrate utilization in men. Am J Clin Nutr 77, 1442–1447.

    Article  CAS  Google Scholar 

  • Kao YH, Hiipakka RA, Liao S (2000). Modulation of endocrine systems and food intake by green tea epigallocatechin gallate. Endocrinology 141, 980–987.

    Article  CAS  Google Scholar 

  • Klaus S, Pultz S, Thone-Reineke C, Wolfram S (2005). Epigallocatechin gallate attenuates diet-induced obesity in mice by decreasing energy absorption and increasing fat oxidation. Int J Obes (Lond) 29, 615–623.

    Article  CAS  Google Scholar 

  • Kovacs EM, Mela DJ (2006). Metabolically active functional food ingredients for weight control. Obes Rev 7, 59–78.

    Article  CAS  Google Scholar 

  • Kovacs EM, Lejeune MP, Nijs I, Westerterp-Plantenga MS (2004). Effects of green tea on weight maintenance after body-weight loss. Br J Nutr 91, 431–437.

    Article  CAS  Google Scholar 

  • Lopez-Garcia E, van Dam RM, Rajpathak S, Willett WC, Manson JE, Hu FB (2006). Changes in caffeine intake and long-term weight change in men and women. Am J Clin Nutr 83, 674–680.

    Article  CAS  Google Scholar 

  • Lorenzen JK, Nielsen S, Holst JJ, Tetens I, Rehfeld JF, Astrup A (2007). Effect of dairy calcium or supplementary calcium intake on postprandial fat metabolism, appetite, and subsequent energy intake. Am J Clin Nutr 85, 678–687.

    Article  CAS  Google Scholar 

  • Magkos F, Kavouras SA (2005). Caffeine use in sports, pharmacokinetics in man, and cellular mechanisms of action. Crit Rev Food Sci Nutr 45, 535–562.

    Article  CAS  Google Scholar 

  • Nagao T, Komine Y, Soga S, Meguro S, Hase T, Tanaka Y et al. (2005). Ingestion of a tea rich in catechins leads to a reduction in body fat and malondialdehyde-modified LDL in men. Am J Clin Nutr 81, 122–129.

    Article  CAS  Google Scholar 

  • Noordzij M, Uiterwaal CS, Arends LR, Kok FJ, Grobbee DE, Geleijnse JM (2005). Blood pressure response to chronic intake of coffee and caffeine: a meta-analysis of randomized controlled trials. J Hypertens 23, 921–928.

    Article  CAS  Google Scholar 

  • Pasman WJ, Westerterp-Plantenga MS, Saris WH (1997). The effectiveness of long-term supplementation of carbohydrate, chromium, fibre and caffeine on weight maintenance. Int J Obes Relat Metab Disord 21, 1143–1151.

    Article  CAS  Google Scholar 

  • Rasmussen DD, Ishizuka B, Quigley ME, Yen SS (1983). Effects of tyrosine and tryptophan ingestion on plasma catecholamine and 3, 4-dihydroxyphenylacetic acid concentrations. J Clin Endocrinol Metab 57, 760–763.

    Article  CAS  Google Scholar 

  • Toubro S, Astrup AV, Breum L, Quaade F (1993). Safety and efficacy of long-term treatment with ephedrine, caffeine and an ephedrine/caffeine mixture. Int J Obes Relat Metab Disord 17 (Suppl 1), S69–S72.

    Google Scholar 

  • Tremblay A, Masson E, Leduc S, Houde A, Després J-P (1988). Caffeine reduces spontaneous energy intake in men but not in women. Nutr Res 8, 553–558.

    Article  CAS  Google Scholar 

  • Weir JB (1949). New methods for calculating metabolic rate with special reference to protein metabolism. J Physiol 109, 1–9.

    Article  Google Scholar 

  • Westerterp-Plantenga MS, Lejeune MP, Kovacs EM (2005). Body weight loss and weight maintenance in relation to habitual caffeine intake and green tea supplementation. Obes Res 13, 1195–1204.

    Article  CAS  Google Scholar 

  • Wolfram S, Raederstorff D, Wang Y, Teixeira SR, Elste V, Weber P (2005). TEAVIGO (epigallocatechin gallate) supplementation prevents obesity in rodents by reducing adipose tissue mass. Ann Nutr Metab 49, 54–63.

    Article  CAS  Google Scholar 

  • Yoshioka M, Doucet E, Drapeau V, Dionne I, Tremblay A (2001). Combined effects of red pepper and caffeine consumption on 24 h energy balance in subjects given free access to foods. Br J Nutr 85, 203–211.

    Article  CAS  Google Scholar 

  • Yoshioka M, St-Pierre S, Drapeau V, Dionne I, Doucet E, Suzuki M et al. (1999). Effects of red pepper on appetite and energy intake. Br J Nutr 82, 115–123.

    CAS  Google Scholar 

  • Zemel MB (2002). Regulation of adiposity and obesity risk by dietary calcium: mechanisms and implications. J Am Coll Nutr 21, 146S–151S.

    Article  CAS  Google Scholar 

  • Zheng G, Sayama K, Okubo T, Juneja LR, Oguni I (2004). Anti-obesity effects of three major components of green tea, catechins, caffeine and theanine, in mice. In vivo 18, 55–62.

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Acknowledgements

We thank John Lind for his expert technical assistance and Jens Kondrup for critical reading of the manuscript. The study was supported by a grant from Science, Toxicology & Technology, San Francisco, CA, USA. The dietary supplements containing the ingredients examined in the present paper were manufactured by Alpine Health Products, Salt Lake City, UT, USA, and are not commercially available.

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  1. Department of Human Nutrition, Faculty of Life Sciences, University of Copenhagen, Copenhagen, Denmark

    A Belza, S Toubro & A Astrup

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  1. A Belza
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Correspondence to A Belza.

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Belza, A., Toubro, S. & Astrup, A. The effect of caffeine, green tea and tyrosine on thermogenesis and energy intake. Eur J Clin Nutr 63, 57–64 (2009). https://doi.org/10.1038/sj.ejcn.1602901

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