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A double-blind placebo-controlled study to establish the bifidogenic dose of inulin in healthy humans

Abstract

Objective:

To evaluate the bifidogenic efficacy of two inulin doses in healthy human adults.

Design:

A double-blind, placebo-controlled, crossover human study.

Setting:

Food Microbial Sciences Unit, The University of Reading, Reading, UK.

Subjects:

Thirty healthy volunteers, 15 men, 15 women (age range 19–35).

Interventions:

Subjects consumed a chocolate drink containing placebo (maltodextrin, 8 g/day), 5 g/day inulin and 8 g/day inulin for a 2-week treatment period. Each treatment was followed by a 1-week washout at the end of which volunteers progressed to the next treatment. Faecal samples were obtained at the start of the study (baseline) and at the end of each treatment and washout period. Fluorescent in situ hybridization was used to monitor populations of Bifidobacterium genus, Bacteroides – Prevotella, Lactobacillus – Enterococcus and Clostridium perfringenshistolyticum subgroup.

Results:

Bifidobacterial levels increased significantly upon ingestion of both the low (9.78±0.29 log10 cells/g faeces, P<0.05) and the high inulin dose (9.79±0.38 log10 cells/g faeces, P=0.05) compared to placebo (9.64±0.23 log10 cells/g faeces).

Conclusions:

Both inulin doses exhibited a bifidogenic effect but a higher volunteer percentage responded to the high dose. A dose response effect was not observed but the magnitude of increase in bifidobacteria levels depended on their initial numbers. The higher the initial concentrations the smaller was the increase upon ingestion of the active treatments.

Sponsorship:

Financial support for the completion of this project was provided by Sensus (Roosendaal, The Netherlands).

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Correspondence to S Kolida.

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Kolida, S., Meyer, D. & Gibson, G. A double-blind placebo-controlled study to establish the bifidogenic dose of inulin in healthy humans. Eur J Clin Nutr 61, 1189–1195 (2007). https://doi.org/10.1038/sj.ejcn.1602636

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  • DOI: https://doi.org/10.1038/sj.ejcn.1602636

Keywords

  • inulin
  • FISH
  • prebiotic
  • colon
  • Bifidobacterium

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