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  • Original Article
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Folate intakes and folate biomarker profiles of pregnant Japanese women in the first trimester

European Journal of Clinical Nutrition volume 61, pages 83–90 (2007)Cite this article

A Corrigendum to this article was published on 04 April 2007

Abstract

Objective:

To assess the status of dietary folate intake, serum and red blood cell (RBC) folate, and related nutritional biomarkers in healthy Japanese women in early pregnancy.

Design:

A cross-sectional, observational study.

Subjects:

Pregnant women in the first trimester, at 7–15 weeks gestation (n=70), who were not consuming any folate supplements or folate fortified foods.

Methods:

Three-day dietary records were obtained from each subject to assess dietary folate intake. Blood samples were collected for measurement of biomarkers. Biomarkers and nutrient intake were analyzed in two groups defined by their serum folate concentrations: the low folate group (serum folate <9 ng/ml) and the high folate group (serum folate ⩾9 ng/ml).

Result:

Mean serum and RBC folate concentrations in all subjects were 10.3 and 519 ng/ml, respectively. These levels were remarkably higher than the reported values from many other countries despite our subjects receiving no folic acids supplements. However, mean folate intake by our subjects from natural foods was 289 μg/day, which is thought to be low according to the Japanese dietary recommendation specified for pregnant women. The intake of spinach and fruits was significantly greater in the high folate group than in the low folate group.

Conclusion:

Folate intake was thought to be adequate to maintain a desirable level of serum folate concentration in Japanese pregnant women in the first trimester, although the intake of folate from natural food was not high enough to meet the recommended daily intake.

Sponsorship:

This study was funded by a Ministry of Health Labour and Welfare, Health and Labour Research Grant, for Research on Children and Families.

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References

  • Berry RJ, Li Z, Erickson JD, Li S, Moore CA, Wang H et al. (1999). Prevention of neural-tube defects with folic acid in China. China–US Collaborative Project for Neural Tube Defect Prevention. N Engl J Med 341, 1485–1490.

    Article  CAS  Google Scholar 

  • Brouwer IA, van Dusseldorp M, West CE, Meyboom S, Thomas CM, Duran M et al. (1999). Dietary folate from vegetables and citrus fruit decreases plasma homocysteine concentrations in humans in a dietary controlled trial. J Nutr 129, 1135–1139.

    Article  CAS  Google Scholar 

  • Brown JE, Jacobs Jr DR, Hartman TJ, Barosso GM, Stang JS, Gross MD et al. (1997). Predictors of red cell folate level in women attempting pregnancy. JAMA 277, 548–552.

    Article  CAS  Google Scholar 

  • Bunduki V, Dommergues M, Zittoun J, Marquet J, Muller F, Dumez Y (1995). Maternal-fetal folate status and neural tube defects: a case control study. Biol Neonate 67, 154–159.

    Article  CAS  Google Scholar 

  • Centers for Disease Control and Prevention (CDC) (2002). Folate status in women of childbearing age, by race/ethnicity – United States, 1999–2000. MMWR Morb Mortal Wkly Rep 51, 808–810.

  • Cuskelly GJ, McNulty H, Scott JM (1996). Effect of increasing dietary folate on red-cell folate: implications for prevention of neural tube defects. Lancet 347, 657–659.

    Article  CAS  Google Scholar 

  • Daly LE, Kirke PN, Molloy A, Weir DG, Scott JM (1995). Folate levels and neural tube defects. Implications for prevention. JAMA 274, 1698–1702.

    Article  CAS  Google Scholar 

  • Green NS (2002). Folic acid supplementation and prevention of birth defects. J Nutr 132, 2356S–2360S.

    Article  CAS  Google Scholar 

  • Gregory III JF (2001). Case study: folate bioavailability. J Nutr 131, 1376S–1382S.

    Article  CAS  Google Scholar 

  • Hirooka M (2001). Nutritional status of vitamin A, E, C, B1, B2, B6, nicotinic acid, B12, folate, and β-carotene in young women. J Nutr Sci Vitaminol 47, 20–27.

    Article  Google Scholar 

  • Hoffbrand AV, Newcombe BFA, Mollin DL (1966). Method of assay of red cell folate activity and the value of the assay as a test for folate deficiency. J Clin Pathol 19, 17–28.

    Article  CAS  Google Scholar 

  • Horne DW (1997). Microbiological assay of folates in 96-well microtiter plates. Methods Enzymol 28, 38–53.

    Article  Google Scholar 

  • Jacques PF, Selhub J, Bostom AG, Wilson PW, Rosenberg IH (1999). The effect of folic acid fortification on plasma folate and total homocysteine concentrations. N Engl J Med 340, 1449–1454.

    Article  CAS  Google Scholar 

  • Jones ML, Nixon PF (2002). Tetrahydrofolates are greatly stabilized by binding to bovine milk folate-binding protein. J Nutr 132, 2690–2694.

    Article  CAS  Google Scholar 

  • Jones ML, Treloar T, Nixon PF (2003). Dietary interactions influence the effects of bovine folate-binding protein on the bioavailability of tetrahydrofolates in rats. J Nutr 133, 489–495.

    Article  CAS  Google Scholar 

  • Kim TH, Yang J, Darling PB, O’Connor DL (2004). A large pool of available folate exists in the large intestine of human infants and piglets. J Nutr 134, 1389–1394.

    Article  CAS  Google Scholar 

  • Kondo A, Kamihira O, Shimosuka Y, Okai I, Gotoh M, Ozawa H (2005). Awareness of the role of folic acid, dietary folate intake and plasma folate concentration in Japan. J Obstet Gynaecol Res 31, 172–177.

    Article  Google Scholar 

  • Koebnick C, Heins UA, Hoffmann I, Dagnelie PC, Leitzmann C (2001). Folate status during pregnancy in women is improved by long-term high vegetable intake compared with the average western diet. J Nutr 131, 733–739.

    Article  CAS  Google Scholar 

  • Melse-Boonstra A, de Bree A, Verhoef P, Bjorke-Monsen AL, Verschuren WM (2002). Dietary monoglutamate and polyglutamate folate are associated with plasma folate concentrations in Dutch men and women aged 20–65 years. J Nutr 132, 1307–1312.

    Article  CAS  Google Scholar 

  • Ministry of Health, Labour, & Welfare, Japan 2001. Kokumin Eiyouno Genjou (Annual Report of the National Nutrition Survey in 2001). Daiichi Publishing: Tokyo. (in Japanese).

  • Ministry of Health, Labour, & Welfare, Japan 2005. Dietary Reference Intakes for Japanese, 2005. Daiichi Publishing: Tokyo. (in Japanese).

  • Rao S, Yajnik CS, Kanade A, Fall CH, Margetts BM, Jackson AA et al. (2001). Intake of micronutrient-rich foods in rural Indian mothers is associated with the size of their babies at birth: Pune Maternal Nutrition Study. J Nutr 131, 1217–1224.

    Article  CAS  Google Scholar 

  • Ray JG, Meier C, Vermeulen MJ, Boss S, Wyatt PR, Cole DE (2002). Association of neural tube defects and folic acid food fortification in Canada. Lancet 360, 2047–2048.

    Article  Google Scholar 

  • Silaste ML, Rantala M, Alfthan G, Aro A, Kesaniemi YA (2003). Plasma homocysteine concentration is decreased by dietary intervention. Br J Nutr 89, 295–301.

    Article  CAS  Google Scholar 

  • Takimoto H, Yoshiike N, Katagiri A, Ishida H, Abe S (2003). Nutritional status of pregnant and lactating women in Japan: a comparison with non-pregnant/non-lactating controls in the National Nutrition Survey. J Obstet Gynaecol Res 29, 96–103.

    Article  CAS  Google Scholar 

  • Watanabe T, Suzuki A, Kumagai M, Kenmoku A, Takeuchi M, Nishimuta M et al. (2003). Applicability of standard tables of food composition in Japan (fifth revised edition) to evaluating the residual components in food after cooking. Jpn J Nutr Diet 61, 251–262.

    Article  Google Scholar 

  • Werler MM, Hayes C, Louik C, Shapiro S, Mitchell AA (1999). Multivitamin supplementation and risk of birth defects. Am J Epidemiol 150, 675–682.

    Article  CAS  Google Scholar 

  • van den Berg H, van der Gaag M, Hendriks H (2002). Influence of lifestyle on vitamin bioavailability. Int J Vitam Nutr Res 72, 53–59.

    Article  CAS  Google Scholar 

Download references

Acknowledgements

We thank Dr Tsunenobu Tamura for the valuable comments in RBC folate assay.

Author information

Authors and Affiliations

  1. Division of Applied Food Research, National Institute of Health and Nutrition, Tokyo, Japan

    N Mito & K Umegaki

  2. Department of Health Promotion and Research, Section of Maternal and Child Health, National Institute of Public Health, Saitama, Japan

    H Takimoto

  3. Division of Health and Nutrition Monitoring, National Institute of Health and Nutrition, Tokyo, Japan

    A Ishiwaki & N Yoshiike

  4. Department of Health Science, Facility of Human Life and Environmental Science, Kochi Women's University, Kochi, Japan

    K Kusama

  5. Department of Developmental Medical Science, Graduate School of International Health, University of Tokyo, Tokyo, Japan

    H Fukuoka

  6. Obstetrics and Gynecology Unit, Ohta Hospital, Tokyo, Japan

    S Ohta

  7. Department of Obstetrics and Gynecology, Metropolitan Ohtsuka Hospital, Tokyo, Japan

    S Abe

  8. Matsushima Women's and Children's Hospital, Tokyo, Japan

    M Yamawaki

  9. Laboratory of Administrative Dietetics, Kagawa Nutrition University, Saitama, Japan

    H Ishida

Authors
  1. N Mito
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  9. M Yamawaki
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Corresponding author

Correspondence to N Mito.

Additional information

Contributors: NM has contributed to data collection and analysis, the main investigation, and writing the paper. HT was involved in planning and coordinating the study, data collection and analysis, and produced the draft of the paper. KU was the supervisor and coordinator of the investigation. AI and KK were involved in data collection and analysis. SA, MY, HF and SO were responsible for selection of patients, and contributed to data collection. HI was involved in the study design. NY designed the study and was the overall supervisor of the project, and approved the manuscript.

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Mito, N., Takimoto, H., Umegaki, K. et al. Folate intakes and folate biomarker profiles of pregnant Japanese women in the first trimester. Eur J Clin Nutr 61, 83–90 (2007). https://doi.org/10.1038/sj.ejcn.1602497

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