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The influence of Iraqi mothers' socioeconomic status on their milk-lipid content

European Journal of Clinical Nutrition volume 60, pages 1400–1405 (2006)Cite this article

Abstract

Objective:

To investigate the lipid content and fatty acid (FA) composition, especially n3 long-chain polyunsaturated fatty acids (n3 LCPUFAs) of mature breast-milk of Iraqi mothers and their relation to the socioeconomic status of the mothers.

Design:

A collection of mature breast-milk of mothers with three different socioeconomic status (lactation period 5±1 month).

Setting:

Mosul province (in the north of Iraq).

Subjects:

Mature breast-milk samples were obtained from a group of 20- to 35-year-old mothers with different socioeconomic status: high urban (HU, n=25), low urban (LU, n=15) and typical suburban (SU, n=25).

Procedure:

Mature-milk samples were collected from each lactating mother. The lipid components of each sample, namely triglycerides (TGs), cholesterol (C) and phospholipids (PLs) were determined enzymatically. After their separation and esterification, FAs were determined as FA methyl esters by capillary gas chromatography.

Results:

The percentages of n3 LCPUFAs were 0.48±0.025, 0.37±0.029 and 0.38±0.018% for HU, LU and SU mothers, respectively. The amount of TGs, the major component of milk lipid, was 5.64±0.24, 5.21±1.61 and 3.21±0.92 g/100 ml for HU, SU and LU mothers, respectively. The milk-lipid content varied with the socioeconomic status.

Conclusion:

The socioeconomic status of lactating mothers affected the lipid content and FA composition, especially the level of n3 LCPUFAs (the very important structural constituents of the retina, brain and other nervous tissues). Mature breast-milk for the studied groups was low in n3 LCPUFAs compared with that of mothers from developed countries and that recommended by WHO for optimum infant nutrition.

Sponsorship:

Supported by grants from Department of Chemistry, University of Mosul. The analysis of FA methyl ester samples was performed at the National Centre for Scientific Research, France.

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References

  • Agostoni C, Marangoni F, Bernardo L, Lammardo AM, Galli C, Riva E (1999). Long-chain polyunsaturated fatty acids in human milk. Acta Paediatr Suppl 88, 68–71.

    Article  CAS  Google Scholar 

  • Ali AM, Al-Shebibi M, Al-Umar ME, Toma SJ (1984). Dairy Chemistry. Mosul University Press: Mosul, Iraq, pp 31–57.

    Google Scholar 

  • Al-Tamer YY, Mahmood AA (2004). Fatty acid composition of colostrum and serum of fullterm and preterm delivering Iraqi mothers. Eur J Clin Nutr 58, 1119–1124.

    Article  CAS  Google Scholar 

  • Al-Tamer YYH (1974). Analysis of lipoprotein fractions separated by density gradient ultracentrifugation. M.Sc. thesis, University of Surrey, UK.

  • Ambartsumyan G (1998). Maternal milk more essential than we think. Noteworthy Nutr Paper 1, 1–4.

    Google Scholar 

  • Beijers RJ, Schaafsma A (1996). Long-chain polyunsaturated fatty acid content in Dutch preterm breast milk; differences in concentrations of docosahexaenoic acid and arachidonic acid due to length of gestation. Early Hum Dev 44, 215–223.

    Article  CAS  Google Scholar 

  • Bitman J, Wood L, Hamosh M, Hamosh P, Mehta NR (1983). Comparison of the lipid composition of breast milk from mothers of term and preterm infants. Am J Clin Nutr 38, 300–312.

    Article  CAS  Google Scholar 

  • Brown KH, Akhtar NA, Robertson AD, Ahmed MG (1986). Lactational capacity of marginally nourished mothers: relationships between maternal nutritional status and quantity and proximate composition of milk. Pediatrics 78, 909–919.

    CAS  Google Scholar 

  • de-Jong C, Bading HT (1990). Determination of free fatty acids in milk and cheese. Procedures of extraction, clean up and capillary gas chromatographic analysis. J High Resol Chrom 13, 94–98.

    Article  CAS  Google Scholar 

  • FAO/WHO (1994). Fats and Oils in Human Nutrition. Report of Joint Expert Consultation, vol. 57. FAO, FAO Food and Nutrition: Rome. pp 1–10.

  • Ferris AM, Jensen RG (1984). Lipids in human milks: a review. 1: Sampling, determination and content. J Pediatr Gastroenterol Nutr 3, 108–122.

    Article  CAS  Google Scholar 

  • Flayeh KA (2000). An Introduction to Biochemistry 2nd edn. Mosul University Press, Mosul, Iraq, pp 64–76.

    Google Scholar 

  • Genzel-Boroviczeny O, Wahle J, Koletzko B (1997). Fatty acid composition of human milk during the first month after term and preterm delivery. Eur J Pediatr 156, 142–147.

    Article  CAS  Google Scholar 

  • Guesnet P, Couet C, Alessandri JM, Antoine JM, Durand G (1995). Variability in linoleic acid (18:2 n6) content and 18:2 n6/18:3 n3 ratio in human breast milk in France. Ann Pediatr 42, 282–286.

    Google Scholar 

  • Harzer G, Dieterich I, Haug M (1984). Effects of the diet on the composition of human milk. Ann Nutr Metab 28, 231–239.

    Article  CAS  Google Scholar 

  • Hawk PB, Oser BL, Summerson WH (1954). Practical Physiological Chemistry. Maple Press Company: New York, pp 31–57.

    Google Scholar 

  • Innis SM (1991). Essential fatty acids in growth and development. Prog Lipid Res 30, 39–103.

    Article  CAS  Google Scholar 

  • Institute of Medicine (1991). Nutrition During Lactation. National Academy Press: Washington, DC, 116 pp.

  • Jensen GL, Jensen RG (1992). Specialty lipids for infant nutrition. II. Concerns, new developments, and future applications. J Pediatr Gastroenterol Nutr 15, 382–394.

    Article  CAS  Google Scholar 

  • Jensen RG (1999). Lipids in human milk. Lipids 34, 1243–1271.

    Article  CAS  Google Scholar 

  • Jensen RG, Lammi-Keefe CJ, Hill DW, Kind AJ, Henderson R (1998). The anticarcinogenic conjugated fatty acid, 9c, 11t-18:2 in human milk: confirmation of its presence. J Hum Lact 14, 23–27.

    Article  CAS  Google Scholar 

  • Jorgensen MH, Lassen A, Michaelsen KF (1995). Fatty acid composition in Danish infant formula compared to human milk. Scand J Nutr 39, 50–54.

    Google Scholar 

  • Kelly ML, Berry JR, Dwyer DA, Griinari JM, Chouinard PY, Van Amburgh ME et al. (1998). Dietary fatty acid sources affect conjugated linoleic acid concentrations in milk from lactating dairy cows. J Nutr 128, 881–885.

    Article  CAS  Google Scholar 

  • Kneebone GM, Kneebone R, Gibson RA (1985). Fatty acid composition of breast milk from three racial groups from Penang, Malaysia. Am J Clin Nutr 41, 765–769.

    Article  CAS  Google Scholar 

  • Krasevec JM, Jones PJ, Cabrera-Hernandez A, Luisa Mayer D, Connor WE (2002). Maternal and infant essential fatty acid status in Havana, Cuba. Am J Clin Nutr 76, 834–844.

    Article  CAS  Google Scholar 

  • Koletzko B, Decsi T (1997). Metabolic aspects of trans fatty acids. Clin Nutr 16, 229–237.

    Article  CAS  Google Scholar 

  • Koletzko B, Thiel I, Abiodun PO (1991). Fatty acid composition of mature human milk in Nigeria. Z Ernahrungswiss 30, 289–297.

    Article  CAS  Google Scholar 

  • Kramer JK, Fellner V, Dugan ME, Sauer FD, Mossoba MM, Yurawecz MP (1997). Evaluating acid and base catalysts in the methylation of milk and rumen fatty acids with special emphasis on conjugated dienes and total trans fatty acids. Lipids 32, 1219–1228.

    Article  CAS  Google Scholar 

  • Laryea MD, Leichsenring M, Mrotzek M, el-Amin EO, el-Kharib AO, Ahmed HM et al. (1995). Fatty acid composition of the milk of well-nourished Sudanese women. Int J Food Sci Nutr 46, 205–214.

    Article  CAS  Google Scholar 

  • Lonnerdal B (1986). Effects of maternal dietary intake on human milk composition. J Nutr 116, 499–513.

    Article  CAS  Google Scholar 

  • Morgan C, Davies L, Corcoran F, Stammers J, Colley J, Spencer SA et al. (1998). Fatty acid balance studies in term infants fed formula milk containing long-chain polyunsaturated fatty acids. Acta Paediatr 87, 136–142.

    Article  CAS  Google Scholar 

  • Muskiet FA, Hutter NH, Martini IA, Jonxis JH, Offringa PJ, Boersma ER (1987). Comparison of the fatty acid composition of human milk from mothers in Tanzania, Curacao and Surinam. Hum Nutr Clin Nutr 41, 149–159.

    CAS  PubMed  Google Scholar 

  • Park Y, Albright KJ, Liu W, Storkson JM, Cook ME, Pariza MW (1997). Effect of conjugated linoleic acid on body composition in mice. Lipids 32, 853–858.

    Article  CAS  Google Scholar 

  • Prentice A, Jarjou LM, Drury PJ, Dewit O, Crawford MA (1989). Breast-milk fatty acids of rural Gambian mothers: effects of diet and maternal parity. J Pediatr Gastroenterol Nutr 8, 486–490.

    Article  CAS  Google Scholar 

  • Rocquelin G, Tapsoba S, Dop MC, Mbemba F, Traissac P, Martin-prevel Y (1998). Lipid content and essential fatty acid (EFA) composition of mature Congolese breast milk are influenced by mothers' nutritional status: Impact on infants' EFA supply. Eur J Clin Nutr 52, 164–171.

    Article  CAS  Google Scholar 

  • Suh M, Wierzbicki AA, Clandinin MT (1994). Dietary fat alters membrane composition in rod outer segments in normal and diabetic rats: impact on content of very-long-chain (C⩾24) polyenoic acids. Biochim Biophys Acta 1214, 54–62.

    Article  CAS  Google Scholar 

  • Tietz NW (1986). Textbook of Clinical Biochemistry. WB Saunders Co.: Philadelphia, USA. p. 135.

    Google Scholar 

  • van Steenbergen WM, Kusin JA, de With C, Lacko E, Jansen AA (1983). Lactation performance of mothers with contrasting nutritional status in rural Kenya. Acta Paediatr Scand 72, 805–810.

    Article  CAS  Google Scholar 

  • Wu FC, Ting YY, Chen HY (2002). Docosahexaenoic acid is superior to eicosapentaenoic acid as the essential fatty acid for growth of grouper, Epinephelus malabaricus. J Nutr 132, 72–79.

    Article  CAS  Google Scholar 

  • Yoneyama K, Goto I, Nagata H, Ikeda J (1994). Effects of maternal food intake on the total protein, fat, lactose and calcium concentrations in human milk. Nippon Koshu Eisei Zasshi 41, 507–517 (abstract).

    CAS  PubMed  Google Scholar 

Download references

Acknowledgements

We thank the French Embassy for granting a 2-month research fellowship without which the analysis of samples by capillary gas chromatography would never have been carried out, as the technique is still not available in Iraq. Also, we express our gratitude to Dr Christian Marazano, Dr Stephane Mons, Dr Alice Olsker and Wafaa Al-Sheikh, the staff of the laboratory of CNRS, France, who provided the facilities for the analysis performed. The cooperation of a large number of volunteers is sincerely acknowledged.

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Authors and Affiliations

  1. Ninevah Medical College, University of Mosul, Mosul, Iraq

    Y Y Al-Tamer

  2. Department of Chemistry, College of Education, University of Mosul, Mosul, Iraq

    A A Mahmood

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  1. Y Y Al-Tamer
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  2. A A Mahmood
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Correspondence to Y Y Al-Tamer.

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Guarantor: YY Al-Tamer.

Contributors: YYA conceived and planned the study, prepared the initial manuscript and wrote the revised version. AAM performed the sampling and all laboratory works, participated in statistical analysis and reading the final manuscript.

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Al-Tamer, Y., Mahmood, A. The influence of Iraqi mothers' socioeconomic status on their milk-lipid content. Eur J Clin Nutr 60, 1400–1405 (2006). https://doi.org/10.1038/sj.ejcn.1602470

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