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Effects of probiotics on the severity of experimental acute pancreatitis

European Journal of Clinical Nutrition volume 60, pages 464–468 (2006)Cite this article

Abstract

Objective:

This study was designed to evaluate the effects of probiotics on the severity of experimental acute pancreatitis.

Design:

Experimental study.

Setting:

Experiments were done in a laboratory at Haydarpasa Numune Teaching and Research Hospital.

Subjects:

A total of 50 Wistar rats were randomly divided into five groups.

Interventions:

Group 1 was control group. Group 2 received an intraperitoneal injection of a 20% solution in 0.15 mol/l NaCL. Group 3 was injected NaCL and fed with probiotics. Acute pancreatitis was induced in rats by intrperitoneal injection of L-Arginine in groups 4 and 5. The rats in group 5 were treated with probiotics. The pancreas was removed for histologic examination. Evaluation of the pathologic changes was done by a new combined histopathologic grading scale.

Results:

The mean scores of fibrosis, acinar cell loss, oedema, parenchymal necrosis, mononuclear cells infiltration, polymorphonuclear leucocytes infiltration, ductal damage and atypical reactive regeneration in group 5 were significantly lower than group 4.

Conclusions:

We demonstrated that enteral feedings with added probiotics can reduce the severity of acute pancreatitis.

Sponsorship:

None.

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References

  • Austrums E, Pupelis G, Snippe K (2003). Postoperative enteral stimulation by gut feeding improves outcomes in severe acute pancreatitis. Nutrition 19, 487–491.

    Article  Google Scholar 

  • Beger HG, Bittner R, Block S, Buchler M (1986). Bacterial contamination of pancreatic necrosis. Gastroenterology 91, 433–438.

    Article  CAS  Google Scholar 

  • Beger HG, Rau B, Mayer J, Pralle U (1997). Natural course of acute pancreatitis. World J Surg 21, 130–135.

    Article  CAS  Google Scholar 

  • Buchler MW, Gloor B, Müller CA, Friess H, Seiler CA, Waldemar U (2000). Acute necrotizing pancreatitis: treatment strategy according to the status of infection. Ann Surg 232, 619–626.

    Article  CAS  Google Scholar 

  • Dugas B, Mercenier A, Lenoir-Wijnkoop I, Arnaud C, Dugas N, Postaire E (1999). Immunity and probiotics. Immunol Today 20, 387–390.

    Article  CAS  Google Scholar 

  • Freiburghaus AU, Redha F, Amman RW (1995). Does acute pancreatitis progress to chronic pancreatitits? A microvascular pancreatitis model in the rat. Pancreas 11, 374–381.

    Article  CAS  Google Scholar 

  • Holzapfel WH, Haberer P, Geisen R, Björrkroth J, Schillinger U (2001). Taxonomy and important features of probiotic microorganisms in food and nutrition. Am J Clin Nutr 73 (Suppl), 365–373.

    Article  Google Scholar 

  • Isenmann R, Rau B, Beger HG (1999). Bacterial infection and extent of necrosis are determinants of organ failure in patients with acute necrotizing pancreatitis. Br J Surg 86, 1020–1024.

    Article  CAS  Google Scholar 

  • Isolauri E, Sütas Y, Kankaanpää P, Arvilommi H, Salminen S (2001). Probiotics: effects on immunity. Am J Clin Nutr 73 (Suppl), 444–450.

    Article  Google Scholar 

  • Kusske AM, Rongione AJ, Reber HA (1996). Cytokines and acute pancreatitis. Gastroenterology 110, 639–642.

    Article  CAS  Google Scholar 

  • Mangiante G, Colucci G, Canepari P, Bassi C, Nicoli N, Casaril A et al. (2001). Lactobacillus plantarum reduces infection of pancreatic necrosis in experimental acute pancreatitis. Dig Surg 18, 47–50.

    Article  CAS  Google Scholar 

  • Marteau PR, de Vrese M, Cellier CJ, Schrezenmeir J (2001). Protection from gastrointestinal disease with the use of probiotics. J Clin Nutr 73 (Suppl), 430–436.

    Article  Google Scholar 

  • Mayer J, Rau B, Gansauge F, Beger HG (2000). Inflammatory mediators in human acute pancreatitis: clinical and pathophysiological implications. Gut 47, 546–552.

    Article  CAS  Google Scholar 

  • Mizunuma T, Kawamura S, Kishino Y (1984). Effects of injecting excess arginine on rat pancreas. J Nutr 114, 467–471.

    Article  CAS  Google Scholar 

  • Nakasaki H, Mitomi T, Tajima T, Ohnishi N, Fuji K (1988). Gut bacterial translocation during total parenteral nutrition in experimental rats and its countermeasure. Am J Surg 175, 38–43.

    Article  Google Scholar 

  • Oláh A, Belágyi T, Issekutz Á, Gamal ME, Bengmark S (2002a). Randomized clinical trial of specific lactobacillus and fibre supplement to early enteral nutrition in patients with acute pancreatitits. Br J Surg 89, 1103–1107.

    Article  Google Scholar 

  • Oláh A, Pardavi G, Belagyi T, Nagy A, Issekkutzz A, Mohammed GE (2002b). Early nasojejunal feeding in acute pancreatitis is associated with a lower complication rate. Nutrition 18, 259–262.

    Article  Google Scholar 

  • Qin HL, Su ZD, Gao Q, Lin QT (2002). Early intrajejunal nutrition: bacterial translocation and gut barrier function of severe acute pancreatitis in dogs. Hepatobiliary Pancreat Dis Int 1, 150–154.

    PubMed  Google Scholar 

  • Sahin M, Ozer S, Vatansev C, Akoz M, Vatansev H, Aksoy F et al (1999). The impact of oral feeding on the severity of acute pancreatitis. Am J Surg 5, 394–398.

    Article  Google Scholar 

  • Tani S, Itoh H, Koide M, Okabayashi Y, Nakamura T, Fujii M et al. (1990). New model of acute pancreatitis induced by excessive does of arginine in rats. Dig Dis Sci 35, 367–374.

    Article  CAS  Google Scholar 

  • Tito JM, Rudnicki M, Jones D, Alpern HD, Gold MS (1993). Peptide YY ameliorates cerulin-induced pancreatic injury in the rat. Am J Surg 165, 690–696.

    Article  CAS  Google Scholar 

Download references

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Authors and Affiliations

  1. The Fourth General Surgical Department of Haydarpasa Numune Teaching and Training Hospital, Uskudar, Istanbul, Turkey

    M A T Muftuoglu, S Isikgor & A Saglam

  2. Pathology Department of Haydarpasa Numune Teaching and Training Hospital, Uskudar, Istanbul, Turkey

    S Tosun

Authors
  1. M A T Muftuoglu
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  2. S Isikgor
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  3. S Tosun
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  4. A Saglam
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Correspondence to M A T Muftuoglu.

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Muftuoglu, M., Isikgor, S., Tosun, S. et al. Effects of probiotics on the severity of experimental acute pancreatitis. Eur J Clin Nutr 60, 464–468 (2006). https://doi.org/10.1038/sj.ejcn.1602338

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  • DOI: https://doi.org/10.1038/sj.ejcn.1602338

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