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  • Original Article
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Phyto-oestrogen intake in Scottish men: use of serum to validate a self-administered food-frequency questionnaire in older men

Abstract

Objective:

To study dietary intake and serum concentrations of isoflavones in order to provide relative validation of isoflavone intake estimates from the Scottish Collaborative Group – Food-Frequency Questionnaire (SCG-FFQ).

Design:

Validation study.

Setting:

Southern Scotland.

Method:

Dietary intake of isoflavones was estimated using the semiquantitative SCG-FFQ and rank correlation and Kappa statistics were used for the relative validation of intakes against serum isoflavone concentrations in 203 male participants who were population controls in a case–control study of diet and prostate cancer.

Results:

The median intake of isoflavones (daidzein and genistein) was 1.0 mg/day (I-QR 0.6–1.8). The median serum concentration of genistein was 33.79 nmol/l (I-QR 14.12–64.93), nearly twice that of daidzein (18.00 nmol/l, I-QR 8.26–29.45). Equol was detected in 49% of subjects; in these subjects the median was 0.67 nmol/l (I-QR 0.34–1.51). Isoflavone intake was significantly correlated with serum concentrations of daidzein (ρ=0.24, P=0.001), genistein (ρ=0.26, P<0.001) and total isoflavonoids (sum of daidzein, genistein and equol) (ρ=0.27, P<0.001). Whereas values for weighted Kappa ranged from 0.16 (P=0.002) for daidzein and equol combined to 0.22 (P<0.001) for genistein.

Conclusions:

These results demonstrate the suitability of the SCG-FFQ to rank usual isoflavone intakes in older Scottish men, a population observed to have low consumption of soy foods.

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References

  • Adlercreutz H, Markkanen H, Watanabe S (1993). Plasma concentrations of phyto-oestrogens in Japanese men. Lancet 342, 1209–1210.

    Article  CAS  Google Scholar 

  • Adlercreutz H, Mazur W (1997). Phyto-oestrogens and Western disease. Ann Med 29, 95–120.

    Article  CAS  Google Scholar 

  • Altman DG (1991). Practical Statistics for Medical Research. Chapman & Hall: London.

    Google Scholar 

  • Arai Y, Uehara M, Sato Y, Kimira M, Eboshida A, Adlercreutz H et al. (2000). Comparison of isoflavones among dietary intake, plasma concentration and urinary excretion for accurate estimation of phytoestrogen intake. J Epidemiol 10, 127–135.

    Article  CAS  Google Scholar 

  • Armstrong B, Doll R (1975). Environmental factors and cancer incidence and mortality in different countries with special reference to dietary practices. Int J Cancer 15, 617–631.

    Article  CAS  Google Scholar 

  • Atkinson C, Skor HE, Fitzgibbons ED, Scholes D, Chen C, Wahala K et al. (2002). Overnight urinary isoflavone excretion in a population of women living in the United States, and its relationship to isoflavone intake.[erratum appears in Cancer Epidemiol Biomarkers Prev 2002 Nov;11(11):1511]. Cancer Epidemiol Bio Prev 11, 253–260.

    CAS  Google Scholar 

  • Black AE, Goldberg GR, Jebb SA, Livingstone MB, Cole TJ, Prentice AM (1991). Critical evaluation of energy intake data using fundamental principles of energy physiology: 2. Evaluating the results of published surveys. [Review] [75 refs]. Eur J Clin Nut 45, 583–599.

    CAS  Google Scholar 

  • Chan W, Brown J, Buss DH (1994). Miscellaneous Foods: Fourth Supplement to the 5th Edition of McCance and Widdowson's The Composition of Foods. Cambridge: Royal Society of Chemistry.

    Google Scholar 

  • Chan W, Brown J, Church SM, Buss DH (1996). Meat Products and Dishes: Sixth Supplement of the 5th Edition of McCance and Widdowson's The Composition of Foods. Cambridge: Royal Society of Chemistry.

    Google Scholar 

  • Chan W, Brown J, Lee SM (1995). Meat, Poultry and Game: Fifth Supplement to the 5th Edition of McCance and Widdowson's The Composition Foods. Cambridge: Royal Society of Chemistry.

    Google Scholar 

  • Chen Z, Zheng W, Custer LJ, Dai Q, Shu X-O, Jin F et al. (1999). Usual dietary consumption of soy foods and it correlation with the excretion rate of isoflavonoids in overnight urine samples among Chinese women in Shanghai. Nutr Cancer 33, 82–87.

    Article  CAS  Google Scholar 

  • Clarke DB, Barnes KA, Castle L, Rose M, Wilson LA, Baxter MJ et al. (2003). Levels of phytoestrogens, incorganic trace-elemnts, natural toxicants and nitrate in vegetarian duplicate diets. Food Chem 81, 287–300.

    Article  CAS  Google Scholar 

  • Clarke DB, Lloyd AS (2004). Dietary exposure estimates of isoflavones from the 1998 UK Total Diet Study. Food Addit Contam 21, 305–316.

    Article  CAS  Google Scholar 

  • Coward L, Barnes NC, Setchell KD, Barnes S (1993). Genistein, daidzein, and their beta-glycoside conjugates – antitumor isoflavones in soybean foods from american and asian diets. J Agric Food Chem 41, 1961–1967.

    Article  CAS  Google Scholar 

  • Frankenfeld CL, Patterson RE, Horner NK, Neuhouser ML, Skor HE, Kalhorn TF et al. (2003). Validation of a soy food-frequency questionnaire and evaluation of correlates of plasma isoflavone concentrations in postmenopausal women. Am J Clin Nutr 77, 674–680.

    Article  CAS  Google Scholar 

  • Frankenfeld CL, Patterson RE, Kalhorn TF, Skor HE, Howald WN, Lampe JW (2002). Validation of a soy food frequency questionnaire with plasma concentrations of isoflavones in US adults. J Am Diet Assoc 102, 1407–1413.

    Article  Google Scholar 

  • Giovannucci E, Rimm EB, Ascherio A, Colditz GA, Spiegelman D, Stampfer HJ et al. (1999). Smoking and risk of total and fatal prostate cancer in United States health professionals. Cancer Epidemiol Bio Prev 8, 277–282.

    CAS  Google Scholar 

  • Goldberg GR, Black AE, Jebb SA, Cole TJ, Murgatroyd PR, Coward WA et al. (1991). Critical evaluation of energy intake data using fundamental principles of energy physiology: 1. Derivation of cut-off limits to identify under-recording. [Review] [39 refs]. Eur J Clin Nut 45, 569–581.

    CAS  Google Scholar 

  • Griffiths K, Denis L, Turkes A, Morton MS (1998). Possible relationship between dietary factors and pathogenesis of prostate cancer. Int J Urol 5, 195–213.

    Article  CAS  Google Scholar 

  • Holland B, Brown J, Buss DH (1993). Fish and Fish Products: Third Supplement to the 5th Edition of McCance and Widdowson's The Composition of Foods. Cambridge: Royal Society of Chemistry.

    Google Scholar 

  • Holland B, Welch A, Buss DH (1988). Cereals and Cereal Products: Third Supplement to the 4th Edition of McCance and Widdowson's The Composition of Foods. Cambridge: Royal Society of Chemistry.

    Google Scholar 

  • Holland B, Welch A, Buss DH (1989). Milk Products and Eggs: Fourth Supplement to the 4th Edition of McCance and Widdowson's The Composition of Foods. Cambridge: Royal Society of Chemistry.

    Google Scholar 

  • Holland B, Welch A, Buss DH (1991a). Vegetables, Herbs and Spices: Fifth Supplement to the 4th Edition of McCance and Widdowson's The Composition of Foods. Cambridge: Royal Society of Chemistry.

    Google Scholar 

  • Holland B, Welch A, Unwin ID, Buss DH, Paul AA, Southgate DAT (1991b). McCance and Widdowson's The Composition of Foods 5th edn, Cambridge: Royal Society of Chemistry.

    Google Scholar 

  • Holland B, Welch A, Buss DH (1992a). Fruit and Nuts: First Supplement to the 5th Edition of McCance and Widdowson's The Composition of Foods. Cambridge: Royal Society of Chemistry.

    Google Scholar 

  • Holland B, Welch A, Buss DH (1992b). Vegetable Dishes: Second Supplement to the 5th Edition of McCance and Widdowson's The Composition of Foods. Cambridge: Royal Society of Chemistry.

    Google Scholar 

  • Horn-Ross PL, Barnes S, Lee M, Coward L, Mandel E, Koo J et al. (2000a). Assessing phytoestrogen exposure in epidemiologic studies: development of a database (United States). Cancer Causes Control 11, 289–298.

    Article  CAS  Google Scholar 

  • Horn-Ross PL, Lee M, John EM, Koo J (2000b). Sources of phytoestrogen exposure among non-Asian women in California, USA. Cancer Causes Control 11, 299–302.

    Article  CAS  Google Scholar 

  • Huang MH, Harrison GG, Mohamed MM, Gornbein JA, Henning SM, Go VLW et al. (2000). Assessing the accuracy of a food frequency questionnaire for estimating usual intake of phytoestrogens. Nutr Cancer 37, 145–154.

    Article  CAS  Google Scholar 

  • Hunter D (1998). Biochemical indicators of dietary intake. In: ed Willett WC (ed). Nutritional Epidemiology. Oxford University Press: New York, pp 174–243.

    Google Scholar 

  • Kirk P, Patterson RE, Lampe J (1999). Development of a soy food frequency questionnaire to estimate isoflavone consumption in US adults. J Am Diet Assoc 99, 558–563.

    Article  CAS  Google Scholar 

  • Kuzer MS, Xu X (1997). Dietary phytoestrogens. Annu Rev Nutr 17, 353–381.

    Article  Google Scholar 

  • Landis J, Koch GG (1977). The measurement of observer agreement for categorical data. Biometrics 33, 159–174.

    Article  CAS  Google Scholar 

  • Liggins J, Bluck LJ, Runswick S, Atkinson C, Coward WA, Bingham SA (2000a). Daidzein and genistein contents of vegetables. Br J Nutr 84, 717–725.

    CAS  PubMed  Google Scholar 

  • Liggins J, Bluck LJC, Runswick S, Atkinson C, Coward WA, Bingham SA (2000b). Daidzein and genistein content of fruits and nuts. J Nutr Biochem 11, 326–331.

    Article  CAS  Google Scholar 

  • Maskarinec G, Singh S, Meng L, Franke AA (1998). Dietary soy intake and urinary isoflavone excretion among women from a multiethnic population. Cancer Epidemiol Bio Prev 7, 613–619.

    CAS  Google Scholar 

  • Masson LF, McNeill G, Tomany JO, Simpson JA, Peace HS, Wei L et al. (2003). Statistical approaches for assessing the relative validity of a food-frequency questionnaire: use of correlation coefficients and the kappa statistic. Public Health Nutr 6, 313–321.

    Article  CAS  Google Scholar 

  • Mazur W (1998). Phytoestrogen content in foods. Bailliere's Clin Endocrinol Metab 12, 729–742.

    Article  CAS  Google Scholar 

  • Morton MS, Arisaka O, Miyake N, Morgan LD, Evans BA (2002). Phytoestrogen concentrations in serum from Japanese men and women over forty years of age. J Nutr 132, 3168–3171.

    Article  CAS  Google Scholar 

  • Morton MS, Chan PS, Cheng C, Blacklock N, Matos-Ferreira A, Abranches-Monteiro L et al. (1997). Lignans and isoflavonoids in plasma and prostatic fluid in men: samples from Portugal, Hong Kong, and the United Kingdom. Prostate 32, 122–128.

    Article  CAS  Google Scholar 

  • Morton MS, Wilcox G, Wahlqvist ML, Griffiths K (1994). Determination of lignans and isoflavonoids in human female plasma following dietary supplementation. J Endocrinol 142, 251–259.

    Article  CAS  Google Scholar 

  • Muir CS, Nectoux J, Staszewski J (1991). The epidemiology of prostatic cancer: geographical distribution and time-trends. Acta Oncol 30, 133–140.

    Article  CAS  Google Scholar 

  • Mulligan AA, Luben RN, Welch AA, Bingham SA (2002). Daidzein and genistein intakes in England (the EPIC Norfolk cohort). IARC Sci Publ 156, 369–370.

    CAS  PubMed  Google Scholar 

  • Murphy PA, Song T, Buseman G, Barua K, Beecher GR, Trainer D, Holden J (1999). Isoflavones in retail and institutional soy foods. J Agric Food Chem 47, 2697–2704.

    Article  CAS  Google Scholar 

  • Parkin DM, Muir CS (1992). Cancer incidence in five continents Vol. VI. comparability and quality of data. IARC Sci Publ 45–173.

  • Ritchie MR, The Isoflavone Database (2004). http://medicine.st-and.ac.uk/research/docs/ritchie/.

  • Ritchie MR, Morton MS, Deighton N, Blake A, Cummings JH (2004a). Plasma and urinary phyto-oestrogens as biomarkers of intake: validation by duplicate diet analysis. Br J Nutr 91, 447–457.

    Article  CAS  Google Scholar 

  • Ritchie MR, Morton MS, Deighton N, Blake A, Steel CM, Cummings JH (2004b). Plasma and urine concentrations of isoflavones as biomarkers of phyto-oestrogen intake following dietary soy supplementation. Evidence-Based Integr Med 1, 101–112.

    Article  Google Scholar 

  • Ritchie MR, Cummings JH, Morton MS, Steel CM, Bolton-Smith C, Riches AC (2005). A newly constructed and validated isoflavone database for the assessment of total genistein and daidzein intake. Br J Nutr (in press).

  • Rowland I, Wiseman H, Sanders T, Adlercreutz H, Bowey E (1999). Metabolism of oestrogens and phytoestrogen: role of the gut microflora. Biochem Soc Trans 27, 304–308.

    Article  CAS  Google Scholar 

  • Rowland IR, Wiseman H, Sanders TAB, Adlercreutz H, Bowey EA (2000). Interindividual variation in metabolism of soy isoflavones and lignans: Influence of habitual diet on equol production by the gut microflora. Nutr Cancer 36, 27–32.

    Article  CAS  Google Scholar 

  • Schofield WN (1985). Predicting basal metabolic rate, new standards and review of previous work. Human Nutr Clin Nutr 39 (Suppl 1), 5–41.

    Google Scholar 

  • Seow A, Shi CY, Franke AA, Hankin JH, Lee HP, Yu MC (1998). Isoflavonoid levels in spot urine are associated with frequency of dietary soy intake in a population-based sample of middle-aged and older Chinese in Singapore. Cancer Epidemiol Bio Prev 7, 135–140.

    CAS  Google Scholar 

  • Setchell KD (1998). Phytoestrogens: the biochemistry, physiology, and implications for human health of soy isoflavones. Am J Clin Nutr 68, 1333S–1346S.

    Article  CAS  Google Scholar 

  • Sharp L, Little J, Brockton N, Cotton SC, Haites NE, Cassidy J (2002). Intake of folate and related micronutrients, genetic polymorphisms in MTHFR and colorectal cancer: A population-based case-control study in Scotland. J Nutr 132, 3542S.

    Google Scholar 

  • Uehara M, Arai Y, Watanabe S, Adlercreutz H (2000). Comparison of plasma and urinary phytoestrogens in Japanese and Finnish women by time-resolved fluoroimmunoassay. Biofactors 12, 217–225.

    Article  CAS  Google Scholar 

  • Verkasalo PK, Appleby PN, Allen NE, Davey G, Adlercreutz H, Key TJ (2001). Soya intake and plasma concentrations of daidzein and genistein: validity of dietary assessment among eighty British women (Oxford arm of the European Prospective Investigation into Cancer and Nutrition). Br J Nutr 86, 415–421.

    Article  CAS  Google Scholar 

  • Wakai K, Egami I, Kato K, Kawamura T, Tamakoshi A, Lin Y et al. (1999). Dietary intake and sources of isoflavones among Japanese. Nutr Cancer 33, 139–145.

    Article  CAS  Google Scholar 

  • Watanabe S, Yamaguchi M, Sobue T, Takahashi T, Miura T, Arai Y et al. (1998). Pharmacokinetics of soybean isoflavones in plasma, urine and faeces of men after ingestion of 60 g baked soybean powder (Kinako). J Nutr 128, 1710–1715.

    Article  CAS  Google Scholar 

  • Yamamoto S, Sobue T, Sasaki S, Kobayashi M, Arai Y, Uehara M et al. (2001). Validity and reproducibility of a self-administered food-frequency questionnaire to assess isoflavone intake in a Japanese population in comparison with dietary records and blood and urine isoflavones. J Nutr 131, 2741–2747.

    Article  CAS  Google Scholar 

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Acknowledgements

We thank Dr Geraldine McNeil and colleagues for their support with the SCG-FFQ, and Polly Sommerville and Morag Leitch for their help with the FFQ scanning and data collection. This study was part of the PCANDIET Study, supported by CSA 4718 from the Food Standards Agency.

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Correspondence to C L Heald.

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Guarantor: C. Heald.

Contributors: CH conducted the study and prepared the first and final draft of the manuscript, CB-S assisted with the methodology and analysis and contributed to the manuscript, MR created the isoflavone database as used in the study and contributed to the manuscript, MM carried out the gas chromatography-mass spectrometry analysis of the samples and contributed to the manuscript, FA assisted with the study design and methodology and contributed to the manuscript.

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Heald, C., Bolton-Smith, C., Ritchie, M. et al. Phyto-oestrogen intake in Scottish men: use of serum to validate a self-administered food-frequency questionnaire in older men. Eur J Clin Nutr 60, 129–135 (2006). https://doi.org/10.1038/sj.ejcn.1602277

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