Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Original Article
  • Published:

Determination of glycaemic index; some methodological aspects related to the analysis of carbohydrate load and characteristics of the previous evening meal

Abstract

Objectives:

To determine the possible differences in glycaemic index (GI) depending on (1) the analytical method used to calculate the ‘available carbohydrate’ load, that is, using carbohydrates by difference (total carbohydrate by difference, minus dietary fibre (DF)) as available carbohydrates vs available starch basis (total starch minus resistant starch (RS)) of a food rich in intrinsic RS and (2) the effect of GI characteristics and/or the content of indigestible carbohydrates (RS and DF) of the evening meal prior to GI testing the following morning.

Design:

Blood glucose and serum insulin responses were studied after subjects consuming (1) two levels of barley kernels rich in intrinsic RS (15.2%, total starch basis) and (2) after a standard breakfast following three different evening meals varying in GI and/or indigestible carbohydrates: pasta, barley kernels and white wheat bread, respectively.

Subjects:

Healthy adults with normal body mass index.

Results:

(1) Increasing the portion size of barley kernels from 79.6 g (50 g ‘available carbohydrates’) to 93.9 g (50 g available starch) to adjust for its RS content did not significantly affect the GI or insulin index (II). (2) The low GI barley evening meal, as opposed to white wheat bread and pasta evening meals, reduced the postprandial glycaemic and insulinaemic (23 and 29%, respectively, P<0.05) areas under the curve at a standardized white bread breakfast fed the following morning.

Conclusion:

(1) Increasing portion size to compensate for the considerable portion of RS in a low GI barley product had no significant impact on GI or II. However, for GI testing, it is recommended to base carbohydrate load on specific analyses of the available carbohydrate content. (2) A low GI barley evening meal containing high levels of indigestible carbohydrates (RS and DF) substantially reduced the GI and II of white wheat bread determined at a subsequent breakfast meal.

This is a preview of subscription content, access via your institution

Access options

Buy this article

Prices may be subject to local taxes which are calculated during checkout

Figure 1
Figure 2
Figure 3
Figure 4

Similar content being viewed by others

References

  • Åkerberg AKE, Liljeberg HGM, Granfeldt YE, Drews A, Björck IM (1998). An in vitro method, based on chewing, to predict resistant starch content in foods allows parallel determination of potentially available starch and dietary fibre. J Nutr 651–659.

    Article  Google Scholar 

  • AOAC (1995). Offical Methods of Analysis, 16th edn. Association of Official Analytical Chemists: Arlington, VA.

  • Asp N-G, Johansson C-G, Hallmer H, Siljeström M (1983). Rapid enzymatic assay of insoluble and soluble dietary fibre. J Agric Food Chem 31, 476–482.

    Article  CAS  Google Scholar 

  • Björck IME (1996). Starch: nutritional aspects. In: Eliasson A-C (ed). Carbohydrates in Food. Marcel-Dekker Inc.: New York. pp 505–554.

    Google Scholar 

  • Björck IME, Liljeberg HGM, Östman EM (2000). Low glycaemic-index foods. Br J Nutr 83, S149–S155.

    Article  Google Scholar 

  • Brand J, Colagiuri S, Crossman S, Allen A, Roberts D, Truswell S (1991). Low-glycemic index foods improve long term glycemic control in NIDDM. Diabetes Care 14, 95–101.

    Article  CAS  Google Scholar 

  • Brand-Miller JC, Thomas M, Swan V, Ahmad ZI, Petocz P, Colagiuri S (2003). Physiological validation of the concept of glycemic load in lean young adults. J Nutr 133, 2728–2732.

    Article  CAS  Google Scholar 

  • Bravata DM, Sanders L, Huang J, Krumholz HM, Olkin I, Gardner CD et al. (2003). Efficacy and safety of low-carbohydrate diets: a systematic review. J Am Med Assoc 289, 1837–1850.

    Article  CAS  Google Scholar 

  • Brouns F, Björck I, Frayn KN, Gibbs AL, Lang V, Slama G, Wolever TMS (2005). Glycaemic index methodology. Nutr Res Rev 18, 145–171.

    Article  CAS  Google Scholar 

  • DelPrato S, Leonetti F, Simonson DC, Sheehan P, Matsuda M, DeFronzo RA (1994). Effect of sustained physiologic hyperinsulinaemia and hyperglycaemia on insulin secretion and insulin sensitivity in man. Diabetologia 37, 1025–1035.

    Article  CAS  Google Scholar 

  • Englyst HN, Kingman SM, Cummings JH (1992). Classification and measurement of nutritionally important starch fractions. Eur J Clin Nutr 46, S33–S50.

    PubMed  Google Scholar 

  • Faisant N, Pianchot V, Koziowski F, Pacouret MP, Colonna P, Champ M (1995). Resistant starch determination adapted to products containing high level od resistent starch. Sci Aliment 15, 83–89.

    CAS  Google Scholar 

  • FAO/WHO (1998). Carbohydrates in human nutrition: report of a joint FAO/WHO expert consultation. FAO Food and Nutrition Paper 66, 1–140.

  • FAO/WHO (2003). Report: Diet, nutrition and the prevention of chronic diseases: report of a joint WHO/FAO expert consultation WHO Technical Report Series, vol. 916.

  • Fontvieille AM, Rizkalla SW, Penfornis A, Acosta M, Bornet FR, Slama G (1992). The use of low glycaemic index foods improves metabolic control of diabetic patients over five weeks. Diabet Med 9, 444–450.

    Article  CAS  Google Scholar 

  • Foster-Powell K, Holt SH, Brand-Miller JC (2002). International table of glycemic index and glycemic load values: 2002. Am J Clin Nutr 76, 5–56.

    Article  CAS  Google Scholar 

  • Granfeldt YE, Björck IME, Hagander B (1991). On the importance of processing conditions, product thickness and egg addition for the glycaemia and hormonal responses to pasta: a comparison with bread made from ‘pasta ingredients’. Eur J Clin Nutr 45, 489–499.

    CAS  PubMed  Google Scholar 

  • Granfeldt YE, Drews A, Björck IME (1995). Arepas made from high-amylose corn flour produce favourably low glucose and insulin responses in healthy humans. J Nutr 125, 459–465.

    CAS  PubMed  Google Scholar 

  • Holm J, Björck IME, Drews A, Asp N-G (1986). A rapid method for the analysis of starch. Starch/Stärke 38, 224–226.

    Article  CAS  Google Scholar 

  • Järvi AE, Karlström BE, Granfeldt YE, Björck IME, Asp N-G, Vessby BOH (1999). Improved glycemic control and lipid profile and nomalized fibrinolytic activity on a low-glycemic index diet in type 2 diabetic patients. Diabetes Care 22, 10–18.

    Article  Google Scholar 

  • Jenkins DJA, Cuff D, Wolever TMS, Knowland D, Thompson L, Cohen Z et al. (1987a). Digestibility of carbohydrate foods in an ileostomate: relationship to dietary fibre, in vitro digestibility and glycemic response. Am J Gastroenterol 82, 709–717.

    CAS  PubMed  Google Scholar 

  • Jenkins DJA, Wolever TMS, Buckley G, Lam KY, Giudici S, Kalmusky J et al. (1988). Low-glycemic-index starchy foods in the diabetic diet. Am J Clin Nutr 48, 248–254.

    Article  CAS  Google Scholar 

  • Jenkins DJA, Wolever TMS, Kalmusky J, Guidici S, Giordano C, Patten R et al. (1987b). Low-glycemic index diet in hyperlipidemia: use of traditional starchy foods. Am J Clin Nutr 46, 66–71.

    Article  CAS  Google Scholar 

  • Jenkins DJA, Wolever TMS, Nineham R, Sarson DL, Bloom SR, Ahern J et al. (1980). Improved glucose tolerance four hours after taking guar with glucose. Diabetologia 19, 21–24.

    Article  CAS  Google Scholar 

  • Liljeberg H, Bjorck I (2000). Effects of a low-glycaemic index spaghetti meal on glucose tolerance and lipaemia at a subsequent meal in healthy subjects. Eur J Clin Nutr 54, 24–28.

    Article  CAS  Google Scholar 

  • Liljeberg HGM, Björck IME (1994). Bioavailability of starch in bread products. Postprandial glucose and insulin responses in healthy subjects and in vitro resistant starch content. Eur J Clin Nutr 48, 151–163.

    CAS  PubMed  Google Scholar 

  • Liljeberg HGM, Kerberg AKE, Björck IME (1999). Effect of the glycemic index and content of indigestible carbohydrates of cereal-based breakfast meals on glucose tolerance at lunch in healthy subjects. Am J Clin Nutr 69, 647–655.

    Article  CAS  Google Scholar 

  • McKeown NM, Meigs JB, Liu S, Saltzman E, Wilson PWF, Jaques PF (2004). Carbohydrate nutrition, insulin resistance, and the prevalence of the metabolic syndrome in the Framingham offspring cohort. Diabetes Care 27, 538–546.

    Article  Google Scholar 

  • Östman EM, Elmståhl HGL, Björck IME (2002). Barley bread containing lactic acid improves glucose tolerance at a subsequent meal in healthy men and women. J Nutr 132, 1173–1175.

    Article  Google Scholar 

  • Östman EM, Liljeberg Elmståhl HGM, Björck IME (2001). Inconsistency between glycemic and insulinemic responses to regular and fermented milk products. Am J Clin Nutr 74, 96–100.

    Article  Google Scholar 

  • Salmerón J, Ascherio A, Rimm EB, Colditz GA, Spiegelman D, Jenkins DJ et al. (1997a). Dietary fibre, glycemic load, and risk of NIDDM in men. Diabetes Care 20, 545–550.

    Article  Google Scholar 

  • Salmerón J, Manson JE, Stampfer MJ, Colditz GA, Wing AL, Willett WC (1997b). Dietary fibre, glycemic load, and risk of non-insulin-dependent diabetes mellitus in women. J Am Med Assoc 277, 472–477.

    Article  Google Scholar 

  • Thorburn A, Muir J, Proitto J (1993). Carbohydrate fermentation lowers hepatic glucose output in healthy subjects. Metabolism 42, 780–785.

    Article  CAS  Google Scholar 

  • Venter CS, Vorster HH, Cummings JH (1990). Effects of dietary propionate on carbohydrate and lipid metabolism in healthy volunteers. Am J Gastroenterol 85, 549–553.

    CAS  PubMed  Google Scholar 

  • Wolever TM, Vorster HH, Björck I, Brand-Miller J, Brighenti F, Mann JI et al. (2003). Determination of the glycaemic index of foods: interlaboratory study. Eur J Clin Nutr 57, 475–482.

    Article  CAS  Google Scholar 

  • Wolever TMS, Bolognesi C (1996). Source and amount of carbohydrate affect postprandial glucose and insulin in normal subjects. J Nutr 126, 2798–2806.

    CAS  PubMed  Google Scholar 

  • Wolever TMS, Brighenti F, Royall D (1989). Effect of rectal infusion of short chain fatty acids in human subjects. Am J Gastroenterol 84, 1027–1037.

    CAS  PubMed  Google Scholar 

  • Wolever TMS, Jenkins DJA, Ocana AM, Rao VA, Collier GR (1988). Second-meal effect: low-glycemic-index foods eaten at dinner improve subsequent breakfast glycemic response. Am J Clin Nutr 48, 1041–1047.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Y Granfeldt.

Additional information

Guarantor: Y Granfeldt.

Contributor: YG and XW carried out the experiments and evaluated the results. IB and YG designed the experiments and were responsible for writing the manuscript.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Granfeldt, Y., Wu, X. & Björck, I. Determination of glycaemic index; some methodological aspects related to the analysis of carbohydrate load and characteristics of the previous evening meal. Eur J Clin Nutr 60, 104–112 (2006). https://doi.org/10.1038/sj.ejcn.1602273

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/sj.ejcn.1602273

Keywords

This article is cited by

Search

Quick links