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A thromboxane effect of a hydroxytyrosol-rich olive oil wastewater extract in patients with uncomplicated type I diabetes



To assess the antioxidant/non-antioxidant effects of a hydroxytyrosol (HT)-rich phenolic extract from olive mill wastewaters administered with a breakfast.

Design, setting and subjects:

Five type I diabetic patients received 25 mg of HT the first day and 12.5 mg/day the following 3 days. Blood sampling was carried out at T0 (baseline) and T4d just before the breakfast+HT administration and at time points 1, 2, 3 and 4 h after T0. Urines (24-h) were collected from T0 to T4d. Baseline HbA1c was generally inferior to 10%, glycemia was within the range 6–24 mmol/l, whereas total cholesterol, HDL-chol and triglycerides were normal.


The major finding was the 46% decrease in the serum TXB2 production after blood clotting at T4d. Plasma vitamin A, E, β-carotene were not changed. Vitamin C tended to increase (P=0.075). Plasma antioxidant capacity was enhanced at T0+1 h only, whereas its main determinants (albumin, bilirubin, uric acid) were not modified. Urinary 8-isoPGF2α levels were highly variable and were not affected significantly by HT administration.


The major effect of HT accounts for an antiaggregating platelet action, leading to a possible prevention of thrombotic and microthrombotic processes.

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This study was a part of the European Union shared-cost research project FAIR CT 97-3039 entitled: Natural antioxidants from olive oil processing waste waters.

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Correspondence to C L Léger.

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Guarantor: CL Léger.

Contributors: CLL and BD drew up the experimental design and were the coordinators of the study. CLL wrote the paper. MAC, FM and EM carried out the assessments on plasma, LDL and urine which was organized by JP Cristol in the Department of Lipid Biochemistry and Oxidant Stress of Lapeyronie Hospital for some of them. MAC revised the paper. LM was responsible for the clinical evaluation and recruitment of patients. He was consulted for drawing up the design.

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Léger, C., Carbonneau, M., Michel, F. et al. A thromboxane effect of a hydroxytyrosol-rich olive oil wastewater extract in patients with uncomplicated type I diabetes. Eur J Clin Nutr 59, 727–730 (2005).

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