Abstract
Objective:
To assess the antioxidant/non-antioxidant effects of a hydroxytyrosol (HT)-rich phenolic extract from olive mill wastewaters administered with a breakfast.
Design, setting and subjects:
Five type I diabetic patients received 25 mg of HT the first day and 12.5 mg/day the following 3 days. Blood sampling was carried out at T0 (baseline) and T4d just before the breakfast+HT administration and at time points 1, 2, 3 and 4 h after T0. Urines (24-h) were collected from T0 to T4d. Baseline HbA1c was generally inferior to 10%, glycemia was within the range 6–24 mmol/l, whereas total cholesterol, HDL-chol and triglycerides were normal.
Results:
The major finding was the 46% decrease in the serum TXB2 production after blood clotting at T4d. Plasma vitamin A, E, β-carotene were not changed. Vitamin C tended to increase (P=0.075). Plasma antioxidant capacity was enhanced at T0+1 h only, whereas its main determinants (albumin, bilirubin, uric acid) were not modified. Urinary 8-isoPGF2α levels were highly variable and were not affected significantly by HT administration.
Conclusion:
The major effect of HT accounts for an antiaggregating platelet action, leading to a possible prevention of thrombotic and microthrombotic processes.
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References
Bermejo Benito P, Diaz Lanza AM, Silvan Sen AM, De Santos Galindez J, Fernandez Matellano L, Sanz Gomez A & Abad Martinez MJ (2000): Effects of some iridoids from plant origin on arachidonic acid metabolism in cellular systems. Planta Med. 66, 324–328.
Bessard J, Cracowski JL, Stanke-Labesque F & Bessard G (2001): Determination of isoprostaglandin F2alpha type III in human urine by gas chromatography-electronic impact mass spectrometry. Comparison with enzyme immunoassay. J. Chromatogr. B. Biomed. Sci. Appl. 754, 333–343.
Birlouez-Aragon I, Delcourt C, Tessier F & Papoz L (2001): Associations of age, smoking habits and diabetes with plasma vitamin C of elderly of the POLA study. Int. J. Vitam. Nutr. Res. 71, 53–59.
Cachia O, Léger CL, Boulot P, Vernet MH, Michel F, Crastes de Paulet A & Descomps B (1995): Red blood cell vitamin E concentrations in fetuses are related to but lower than those in mothers during gestation. Am. J. Obstet. Gynecol. 173, 42–51.
Cartron E, Fouret G, Carbonneau MA, Lauret C, Michel F, Monnier L, Descomps B & Léger CL (2003): Red-wine beneficial long-term effect on lipids but not on antioxidant characteristics in plasma in a study comparing three types of wine — description of two O-methylated derivatives of gallic acid in humans. Free Radic. Res. 37, 1021–1035.
De la Puerta R, Gutierrez VR & Hoult JRS (1999): Inhibition of leukocyte 5-lipoxygenase by phenolics from virgin olive oil. Biochem. Pharmacol. 57, 445–449.
Esti M, Cinquanta L & La Notte E (1998): Phenolic compounds in different olive varieties. J. Agric. Food Chem. 46, 32–35.
Feener EP & King GL (1997): Vascular dysfunction in diabetes mellitus. Lancet 350, S9–S13.
Fito M, Covas MI, Lamuela-Raventos RM, Vila J, Torrents J, De La Torre C & Marrugat J (2000): Protective effect of olive oil and its phenolic compounds against low density lipoprotéine oxidation. Lipids 35, 633–638.
Kajita K, Ishizuka T, Miura A, Kanoh Y, Ishizawa M, Kimura M & Yasuda K (2001): Increased platelet aggregation in diabetic patients with microangiopathy despite good glycemic control. Platelets 12, 343–351.
Léger CL (2003): L’huile d’olive: sa place dans l’alimentation humaine. In Lipides et corps gras alimentaires, ed. J Graille, Paris, New York: Lavoisier, Londres. pp 81–105.
Léger CL, Galli C, Visioli F, Carbonneau MA, Michel F, Mas E, Cristol JP, Descomps B & Monnier L (2002): Hydroxytyrosol-enriched olive mill wastewater preparation (HTROW) decreases blood TXB2 and does not change urinary isoprostane production in healthy or diabetic subjects in two parallel intervention studies. Free Radic. Biol. Med. 33, S1–S295.
Léger CL, Kadiri-Hassani N & Descomps B (2000): Decreased superoxide anion production in cultured human promonocyte cells (THP-1) due to polyphenol mixtures from olive oil processing wastewaters. J. Agric. Food Chem. 48, 5061–5067.
Masella R, Giovanni C, Vari R, Di Benetto R, Coni E, Volpe R, Fraone N & Bucci A (2001): Effects of dietary virgin olive oil phenols on low density lipoprotein oxidation in hyperlipidemic patients. Lipids 36, 1195–1202.
Miro-Casas E, Albaladejo MF, Covas MI, Rodriguez JO, Colomer EM, Lamuela Raventos RM & De La Torre R (2001): Capillary gas chromatography-mass spectrometry quantitative determination of hydroxytyrosol and tyrosol in human urine after olive oil intake. Anal. Biochem. 294, 63–72.
Patrono C, Ciabattoni G, Pugliese F, Pinca E, Castrucci G, De Salvo A, Satta MA & Parachini M (1980): Radioimmunoassay of serum thromboxane B2: a simple method of assessing pharmacologic effects on platelet function. Advances Prostagland. Thromb. Res. 6, 187–191.
Petroni A, Blasevich M, Salami M, Papini N, Montedoro GF & Galli C (1995): Inhibition of platelet aggregation and eicosanoid production by phenolic components of olive oil. Thromb. Res. 78, 151–160.
Rice-Evans CA (2000): Measurement of total antioxidant activity as a marker of antioxidant status in vivo: procedures and limitations. Free Radic. Res. 33, S59–S66.
Romani A, Mulinacci N, Pinelli P, Vincieri FF & Cimato A (1999): Polyphenolic content in five tuscany cultivars of Olea europaea L. J. Agric. Food Chem. 47, 964–967.
Shafiee M, Carbonneau MA, Urban N, Descomps B & Léger CL (2003): Grape and grape seed extract capacities at protecting LDL against oxidation generated by Cu2+, AAPH or SIN-1 and at decreasing superoxide THP-1 cell production. A comparison to other extracts or compounds. Free Radic. Res. 37, 573–584.
Soler-Rivas C, Espin JC & Wichers J (2000): Oleuropein and related compounds. J. Sci. Food Agric. 80, 1013–1023.
Visioli F, Bellomo G & Galli C (1998): Free-radical-scavenging properties of olive oil polyphenols. Biochem. Biophys. Res. Commun. 247, 60–64.
Visioli F, Caruso D, Galli C, Viappiani S, Galli G & Sala A (2000a): Olive oils rich in natural catecholic phenols decrease isoprostane excretion in humans. Biochem. Biophys. Res. Communs. 278, 797–799.
Visioli F, Caruso D, Grande S, Bosisio R, Villa M, Galli G, Sirtori C & Galli C (2004): Virgin Olive Oil Study (VOLOS): vasoprotective potential of extra virgin olive oil in mildly dyslipidemic patients. Eur. J. Clin. Nutr. 6, 1–7.
Visioli F, Caruso D, Plasmati E, Patelli R, Mulinacci N, Romani A, Galli G & Galli C (2000b): Hydroxytyrosol, as a component of olive mill waste water, is dose-dependently absorbed and increases the antioxidant capacity of rat plasma. Free Radic. Res. 34, 301–305.
Visioli F & Galli C (2001): Phenolics from olive oil and its waste products. Biological activities in in vitro and in vivo studies. World Rev. Nutr. Diet. 88, 233–237.
Visioli F, Galli C, Grande S, Colonnelli K, Patelli C, Galli G & Caruso D (2003): Hydroxytyrosol excretion differs between rats and humans and depends on the vehicle of administration. J. Nutr. 133, 2612–2615.
Visioli F, Romani A, Mulinacci N, Zarini S, Conte D, Vincieri FF & Galli C (1999): Antioxidant and other biological activities of olive mill waste waters. J. Agric. Food Chem. 47, 3397–3401.
Acknowledgements
This study was a part of the European Union shared-cost research project FAIR CT 97-3039 entitled: Natural antioxidants from olive oil processing waste waters.
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Guarantor: CL Léger.
Contributors: CLL and BD drew up the experimental design and were the coordinators of the study. CLL wrote the paper. MAC, FM and EM carried out the assessments on plasma, LDL and urine which was organized by JP Cristol in the Department of Lipid Biochemistry and Oxidant Stress of Lapeyronie Hospital for some of them. MAC revised the paper. LM was responsible for the clinical evaluation and recruitment of patients. He was consulted for drawing up the design.
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Léger, C., Carbonneau, M., Michel, F. et al. A thromboxane effect of a hydroxytyrosol-rich olive oil wastewater extract in patients with uncomplicated type I diabetes. Eur J Clin Nutr 59, 727–730 (2005). https://doi.org/10.1038/sj.ejcn.1602133
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DOI: https://doi.org/10.1038/sj.ejcn.1602133
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