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Effect of dietary fatty acids on the postprandial fatty acid composition of triacylglycerol-rich lipoproteins in healthy male subjects

European Journal of Clinical Nutrition volume 59, pages 24–34 (2005)Cite this article

Abstract

Objective: The aim of the present study was to investigate the effect of trans-18:1 isomers compared to other fatty acids, especially saturates, on the postprandial fatty acid composition of triacylglycerols (TAG) in chylomicrons and VLDL.

Design: A randomised crossover experiment where five interesterified test fats with equal amounts of palmitic acid (P fat), stearic acid (S fat), trans-18:1 isomers (T fat), oleic acid (O fat), or linoleic acid (L fat) were tested.

Subjects: A total of 16 healthy, normolipidaemic males (age 23±2 y) were recruited.

Interventions: The participants ingested fat-rich test meals (1 g fat per kg body weight) and the fatty acid profiles of chylomicron and VLDL TAG were followed for 8 h.

Results: The postprandial fatty acid composition of chylomicron TAG resembled that of the ingested fats. The fatty acids in chylomicron TAG were randomly distributed among the three positions in accordance with the distributions in test fats. Calculations of postprandial TAG concentrations from fatty acid data revealed increasing amounts up to 4 h but lower response curves (IAUC) for the two saturated fats in accordance with previous published data. The T fat gave results comparable to the O and L fats. The test fatty acids were much less reflected in VLDL TAG and there was no dietary influence on the response curves.

Conclusions: The fatty acid composition in the test fats as well as the positional distributions of these were maintained in the chylomicrons. No specific clearing of chylomicron TAG was observed in relation to time.

Sponsorship: Danish Research Development Program for Food Technology.

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References

  • Bonanome A & Grundy SM (1989): Intestinal absorption of stearic acid after consumption of high fat meals in humans. J. Nutr. 119, 1556–1560.

    Article  CAS  Google Scholar 

  • Brunzell JD, Hazzard WR, Porte D & Bierman EL (1973): Evidence for a common, saturable, triglyceride removal mechanism for chylomicrons and very low density lipoproteins in man. J. Clin. Invest. 52, 1578–1585.

    Article  CAS  Google Scholar 

  • Carriere F, Barrowman JA, Verger R & Laugier R (1993): Secretion and contribution to lipolysis of gastric and pancreatic lipases during a test meal in humans. Gastroenterology 105, 876–888.

    Article  CAS  Google Scholar 

  • Chen Y-DI & Reaven GM (1991): Intestinally-derived lipoproteins: metabolism and clinical significance. Diabetes Metab. Rev. 7, 191–208.

    Article  CAS  Google Scholar 

  • Christie WW (1982): Structural analysis of lipids by means of enzymatic hydrolysis. In Lipid Analysis ed. WW Christie, pp 155–166. Oxford: Pergamon Press.

    Google Scholar 

  • Cohn JS, McNamara JR, Cohn SD, Ordovas JM & Schaefer EJ (1988): Plasma apolipoprotein changes in the triglyceride-rich lipoprotein fraction of human subjects fed a fat-rich meal. J. Lipid Res. 29, 925–936.

    CAS  PubMed  Google Scholar 

  • Cohn JS, McNamara JR, Krasinski SD, Russell RM & Schaefer EJ (1989): Role of triglyceride-rich lipoproteins from the liver and intestine in the etiology of postprandial peaks in plasma triglyceride concentration. Metabolism 38, 484–490.

    Article  CAS  Google Scholar 

  • de Bruin TWA, Brouwer CB, van Linde-Sibenius Trip M, Jansen H & Erkelens DW (1993): Different postprandial metabolism of olive oil and soybean oil: a possible mechanism of the high-density lipoprotein conserving effect of olive oil. Am. J. Clin. Nutr. 58, 477–483.

    Article  CAS  Google Scholar 

  • Gatto LM, Sullivan DR & Samman S (2003): Postprandial effects of dietary trans fatty acids on apolipoprotein(a) and cholesteryl ester transfer. Am. J. Clin. Nutr. 77, 1119–1124.

    Article  CAS  Google Scholar 

  • Gibney MJ & Daly E (1994): The incorporation of n-3 polyunsaturated fatty acids into plasma lipid and lipoprotein fractions in the postprandial phase in healthy volunteers. Eur. J. Clin. Nutr. 48, 866–872.

    CAS  PubMed  Google Scholar 

  • Groot PHE, van Stiphout W-AHJ, Krauss XH, Jansen H & van Tol A (1991): Postprandial lipoprotein metabolism in normolipidemic men with and without coronary artery disease. Arterioscler. Thromb. 11, 653–662.

    Article  CAS  Google Scholar 

  • Hunter KA, Crosbie LC, Horgan GW, Miller GJ & Dutta-Roy AK (2001): Effect of diets rich in oleic acid, stearic acid and linoleic acid on postprandial haemostatic factors in young healthy men. Br. J. Nutr. 86, 207–215.

    CAS  Google Scholar 

  • Jensen J, Bysted A, Dawids S, Hermansen K & Hølmer G (1999): The effect of palm oil, lard, and puff-pastry margarine on postprandial lipid and hormone responses in normal-weight and obese young women. Br. J. Nutr. 82, 469–479.

    CAS  PubMed  Google Scholar 

  • Jones AE, Stolinski M, Smith RD, Murphy JL & Wootton SA (1999): Effect of fatty acid chain length and saturation on the gastrointestinal handling and metabolic disposal of dietary fatty acids in women. Br. J. Nutr. 81, 37–43.

    Article  CAS  Google Scholar 

  • Judd JT, Clevidence BA, Muesing RA, Wittes J, Sunkin ME & Podczasy JJ (1994): Dietary trans-fatty acids: effects on plasma lipids and lipoproteins of healthy men and women. Am. J. Clin. Nutr. 59, 861–868.

    Article  CAS  Google Scholar 

  • Karpe F & Hultin M (1995): Endogenous triglyceride-rich lipoproteins accumulate in rat plasma when competing with a chylomicron-like triglyceride emulsion for a common lipolytic pathway. J. Lipid Res. 36, 1557–1566.

    CAS  PubMed  Google Scholar 

  • Karpe F, Steiner G, Uffelman K, Olivecrona T & Hamsten A (1994): Postprandial lipoproteins and progression of coronary atherosclerosis. Atherosclerosis 106, 83–97.

    Article  CAS  Google Scholar 

  • Lichtenstein AH, Ausman LM, Carrasco W, Jenner JL, Ordovas JM & Schaefer EJ (1993): Hydrogenation impairs the hypolipidemic effect of corn oil in humans. Arterioscler. Thromb. 13, 154–161.

    Article  CAS  Google Scholar 

  • Mackness MI & Durrington PN (1992): Lipoprotein separation and analysis for clinical studies. In Lipoprotein Analysis. A Practical Approach, eds. CA Converse & ER Skinner, pp 1–42. Oxford: IRL Press.

    Google Scholar 

  • Matthews JNS, Altman DG, Campbell MJ & Royston P (1990): Analysis of serial measurements in medical research. BMJ 300, 230–235.

    Article  CAS  Google Scholar 

  • Mattson FH & Volpenhein RA (1964): The digestion and absorption of triglycerides. J. Biol. Chem. 239, 2772–2777.

    CAS  PubMed  Google Scholar 

  • Mensink RP, Zock PL, Katan MB & Hornstra G (1992): Effect of dietary cis- and trans-fatty acids on serum lipoprotein(a) levels in humans. J. Lipid Res. 33, 1493–1501.

    CAS  PubMed  Google Scholar 

  • Mero N, Syvänne M, Rosseneu M, Labeur C, Hilden H & Taskinen M-R (1998): Comparison of three fatty meals in healthy normolipidaemic men: high post-prandial retinyl ester response to soybean oil. Eur. J. Clin. Invest. 28, 407–415.

    Article  CAS  Google Scholar 

  • Muesing RA, Griffin P & Mitchell P (1995): Corn oil and beef tallow elicit different postprandial responses in triglycerides and cholesterol, but similar changes in constituents of high-density lipoproteins. J. Am. Coll. Nutr. 14, 53–60.

    Article  CAS  Google Scholar 

  • Nestel P, Noakes M, Belling B, McArthur R, Clifton P, Janus E & Abbey M (1992): Plasma lipoprotein lipid and Lp(a) changes with substitution of elaidic acid for oleic acid in the diet. J. Lipid Res. 33, 1029–1036.

    CAS  PubMed  Google Scholar 

  • Nestel PJ, Noakes M, Belling GB, McArthur R & Clifton PM (1995): Effect on plasma lipids of interesterifying a mix of edible oils. Am. J. Clin. Nutr. 62, 950–955.

    Article  CAS  Google Scholar 

  • Oomen CM, Ocké MC, Feskens EJM, van Erp-Baart M-AJ, Kok FJ & Kromhout D (2001): Association between trans fatty acid intake and 10-year risk of coronary heart disease in the Zutphen Elderly Study: a prospective population-based study. Lancet 357, 746–751.

    Article  CAS  Google Scholar 

  • Patsch JR, Miesenböck G, Hopferwieser T, Mühlberger V, Knapp E, Dunn JK, Gotto AM & Patsch W (1992): Relation of triglyceride metabolism and coronary artery disease. Studies in the postprandial state. Arterioscler. Thromb. 12, 1336–1345.

    Article  CAS  Google Scholar 

  • Phillips NR, Waters D & Havel RJ (1993): Plasma lipoproteins and progression of coronary artery disease evaluated by angiography and clinical events. Circulation 88, 2762–2770.

    Article  CAS  Google Scholar 

  • Potts JL, Fisher RM, Humphreys SM, Coppack SW, Gibbons GF & Frayn KN (1991): Peripheral triacylglycerol extraction in the fasting and post-prandial states. Clin. Sci. 81, 621–626.

    Article  CAS  Google Scholar 

  • Redgrave TG, Kodali DR & Small DM (1988): The effect of triacyl-sn-glycerol structure on the metabolism of chylomicrons and triacylglycerol-rich emulsions in the rat. J. Biol. Chem. 263, 5118–5123.

    CAS  PubMed  Google Scholar 

  • Roche HM, Zampelas A, Jackson KG, Williams CM & Gibney MJ (1998): The effect of test meal monounsaturated fatty acid:saturated fatty acid ratio on postprandial lipid metabolism. Br. J. Nutr. 79, 419–424.

    Article  CAS  Google Scholar 

  • Salomaa V, Rasi V, Pekkanen J, Jauhiainen M, Vahtera E, Pietinen P, Korhonen H, Kuulasmaa K & Ehnholm C (1993): The effects of saturated fat and n-6 polyunsaturated fat on postprandial lipemia and hemostatic activity. Atherosclerosis 103, 1–11.

    Article  CAS  Google Scholar 

  • Sanders TAB, de Grassi T, Miller GJ & Morrissey JH (2000): Influence of fatty acid chain length and cis/trans-isomerization on postprandial lipemia and factor VII in healthy subjects (postprandial lipids and factor VII). Atherosclerosis 149, 413–420.

    Article  CAS  Google Scholar 

  • Sanders TAB, Oakley FR, Cooper JA & Miller GJ (2001): Influence of a stearic acid-rich structured triacylglycerol on postprandial lipemia, factor VII concentrations, and fibrinolytic activity in healthy subjects. Am. J. Clin. Nutr. 73, 715–721.

    Article  CAS  Google Scholar 

  • Schneeman BO, Kotite L, Todd KM & Havel RJ (1993): Relationship between the responses of triglyceride-rich lipoproteins in blood plasma containing apolipoproteins B-48 and B-100 to a fat-containing meal in normolipidemic humans. Proc. Natl. Acad. Sci. USA 90, 2069–2073.

    Article  CAS  Google Scholar 

  • Shishehbor F, Roche HM & Gibney MJ (1998): The effect of acute carbohydrate load on the monophasic and biphasic nature of the postprandial lipaemic response to acute fat ingestion in human subjects. Br. J. Nutr. 80, 411–418.

    CAS  PubMed  Google Scholar 

  • Simons LA, Dwyer T, Simons J, Bernstein L, Mock P, Poonia NS, Balasubramaniam S, Baron D, Branson J, Morgan L & Roy P (1987): Chylomicrons and chylomicron remnants in coronary artery disease: a case-control study. Atherosclerosis 65, 181–189.

    Article  CAS  Google Scholar 

  • Small DM (1991): The effects of glyceride structure on absorption and metabolism. Annu. Rev. Nutr. 11, 413–434.

    Article  CAS  Google Scholar 

  • Summers LKM, Fielding BA, Ilic V, Quinlan PT & Frayn KN (1998): The effect of triacylglycerol-fatty acid distribution on postprandial metabolism in subcutaneous adipose tissue. Br. J. Nutr. 79, 141–147.

    Article  CAS  Google Scholar 

  • Summers LKM, Fielding BA, Herd SL, Ilic V, Clark ML, Quinlan PT & Frayn KN (1999): Use of structured triacylglycerols containing predominantly stearic and oleic acids to probe early events in metabolic processing of dietary fat. J. Lipid Res. 40, 1890–1898.

    CAS  PubMed  Google Scholar 

  • Tholstrup T, Sandström B, Hermansen JE & Hølmer G (1998): Effect of modified dairy fat on postprandial and fasting plasma lipids and lipoproteins in healthy young men. Lipids 33, 11–21.

    Article  CAS  Google Scholar 

  • Tholstrup T, Sandström B, Bysted A & Hølmer G (2001): Effect of 6 dietary fatty acids on the postprandial lipid profile, plasma fatty acids, lipoprotein lipase, and cholesterol ester transfer activities in healthy young men. Am. J. Clin. Nutr. 73, 198–208.

    Article  CAS  Google Scholar 

  • Willett WC, Stampfer MJ, Manson JE, Colditz GA, Speizer FE, Rosner BA, Sampson LA & Hennekens CH (1993): Intake of trans-fatty acids and risk of coronary heart disease among women. Lancet 341, 581–585.

    Article  CAS  Google Scholar 

  • Wood R, Kubena K, O'Brian B, Tseng S & Martin G (1993): Effect of butter, mono- and polyunsaturated fatty acid-enriched butter, trans-fatty acid margarine, and zero trans-fatty acid margarine on serum lipids and lipoproteins in healthy men. J. Lipid Res. 34, 1–11.

    CAS  PubMed  Google Scholar 

  • Yli-Jokipii K, Kallio H, Schwab U, Mykkänen H, Kurvinen J-P, Savolainen MJ & Tahvonen R (2001): Effect of palm oil and transesterified palm oil on chylomicron and VLDL triacylglycerol structures and postprandial lipid response. J. Lipid Res. 42, 1618–1625.

    CAS  PubMed  Google Scholar 

  • Zampelas A, Williams CM, Morgan L & Wright J (1994): The effect of triacylglycerol fatty acid positional distribution on postprandial plasma metabolite and hormone responses in normal adult men. Br. J. Nutr. 71, 401–410.

    Article  CAS  Google Scholar 

  • Zampelas A, Roche H, Knapper JME, Jackson KG, Tornaritis M, Hatzis C, Gibney MJ, Kafatos A, Gould BJ, Wright J & Williams CM (1998): Differences in postprandial lipaemic response between Northern and Southern Europeans. Atherosclerosis 139, 83–93.

    Article  CAS  Google Scholar 

  • Zilversmith DB (1979): Atherogenesis: a postprandial phenomenon. Circulation 60, 473–485.

    Article  Google Scholar 

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Acknowledgements

The present study was financed by the Danish Research Development Program for Food Technology (FØTEK 2). We thank Marianne Rosenberg, Lis Christensen, and Karen Rasmussen for their excellent technical assistance.

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Author notes

  1. B Sandström: Deceased.

Authors and Affiliations

  1. BioCentrum-DTU, Section of Biochemistry and Nutrition, Center of Advanced Food Studies, The Technical University of Denmark, Lyngby, Denmark

    A Bysted, G Hølmer & P Lund

  2. Research Department of Human Nutrition, Center of Advanced Food Studies, The Royal Veterinary and Agricultural University, Frederiksberg C, Denmark

    B Sandström & T Tholstrup

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  1. A Bysted
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  3. P Lund
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Correspondence to A Bysted.

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Guarantors: A Bysted and G Hølmer.

Contributors: TT, BS, GH, and AB were involved in designing the study. AB analysed all blood samples and prepared chylomicron fractions for determination of the positional distributions. AB performed the statistical calculations and analyses, wrote the original manuscript and edited all subsequent versions. GH and PL supervised the analyses. GH contributed to the original and subsequent manuscripts. TT and BS performed the human trial, prepared lipoprotein fractions for fatty acid analyses, and revised the manuscript.

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Bysted, A., Hølmer, G., Lund, P. et al. Effect of dietary fatty acids on the postprandial fatty acid composition of triacylglycerol-rich lipoproteins in healthy male subjects. Eur J Clin Nutr 59, 24–34 (2005). https://doi.org/10.1038/sj.ejcn.1602028

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