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The effect of vegetable oil-based cheese on serum total and lipoprotein lipids


Objective: To investigate the effect of rapeseed oil-based cheese (milk-fat substituted by rapeseed oil) on serum total and lipoprotein lipid concentrations and blood pressure in reference to ordinary, milk-fat-based cheese in subjects with mildly to moderately elevated serum cholesterol concentration.

Design: Randomized, controlled, single-blind, cross-over clinical trial.

Settings: Outpatient dietary intervention with free-living subjects in Eastern Finland.

Interventions: The study began with a 2 week pre-trial period followed by two 4 week intervention periods. During the intervention study subjects replaced their ordinary cheese or cold cuts with 65 g of rapeseed oil-based or milk-fat-based control cheese. The type of test cheese was switched at 4 weeks of intervention. Altogether 31 subjects completed the study.

Results: Compared with the control cheese period the mean serum total cholesterol concentration was 6.7% (95% Cl −9.9 to −3.5%) lower after 2 weeks and 5.0% (95% Cl −7.5 to −2.5%) lower after 4 weeks of use of rapeseed oil-based cheese. Respectively, LDL cholesterol concentration was 7.0% (95% Cl −11.7 to −2.6%) lower after 2 weeks use and 6.4% (95% Cl −10.0 to −2.8%) lower after 4 weeks' use of rapeseed oil-based cheese.

Conclusions: The present study showed that the rapeseed oil-based cheese reduces serum total and LDL cholesterol concentrations in mildly to moderately hypercholesterolemic subjects when replacing ordinary milk-fat-based cheese in the diet.

Sponsorship: Mildola Ltd, Tuusula, Finland and Kyrönmaan Juustomestarit Ltd, lsokyrö, Finland.

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  • Assmann G, Cullen P, Jossa F, Lewis B, Mancini M . 1999 Coronary heart disease: reducing the risk: the scientific background to primary and secondary prevention of coronary heart disease. A worldwide view. International Task force for the Prevention of Coronary Heart disease Arterioscler. Thromb. Vasc. Biol. 19: 1819–1824

    Article  CAS  Google Scholar 

  • Bierenbaum ML, Reichstein RP, Watkins TR, Maginnis WP, Geller M . 1991 Effects of canola oil on serum lipids in humans J. Am. Coll. Nutr. 10: 228–233

    Article  CAS  Google Scholar 

  • Davis PA, Platon JF, Gershwin ME, Halpern GM, Keen CL, DiPaolo D, Alexander J, Ziboh VA . 1993 A linoleate-enriched cheese product reduces low-density lipoprotein in moderately hypercholesterolemic adults Ann. Intern. Med. 119: 555–559

    Article  CAS  Google Scholar 

  • During A, Combe N, Mazette S, Entressangles B . 2000 Effects on cholesterol balance and LDL cholesterol in the fat of a soft-ripened cheese containing vegetable oils J. Am. Coll. Nutr. 19: 458–466

    Article  CAS  Google Scholar 

  • Friedewald WT, Levy RI, Fredrickson DS . 1972 Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge Clin. Chem. 18: 499–502

    CAS  Google Scholar 

  • Hegsted DM, Ausman LM, Johnson JA, Dallal GE . 1993 Dietary fat and serum lipids: an evaluation of the experimental data Am. J. Clin. Nutr. 57: 875–883

    Article  CAS  Google Scholar 

  • Hendriks HF, Weststrate JA, van Vliet T, Meijer GW . 1999 Spreads enriched with three different levels of vegetable oil sterols and the degree of cholesterol lowering in normocholesterolaemic and mildly hypercholesterolaemic subjects Eur. J. Clin. Nutr. 53: 319–327

    Article  CAS  Google Scholar 

  • Howell WH, McNamara DJ, Tosca MA, Smith BT, Gaines JA . 1997 Plasma lipid and lipoprotein responses to dietary fat and cholesterol: a meta-analysis Am. J. Clin. Nutr. 65: 1747–1764

    Article  CAS  Google Scholar 

  • Keys A, Anderson JT, Grande F . 1957 Prediction of serum-cholesterol responses of man to changes in the diet Lancet 2: 959–966 Referenced in Howell et al, 1997

    Article  Google Scholar 

  • King L . 1999 Dairy products Food Processing 68: 10–11

    Google Scholar 

  • Lichtenstein AH, Ausman LM, Carrasco W, Jenner JL, Gualtieri LJ, Goldin BR, Ordovas JM, Schaefer EJ . 1993 Effects of canola, corn, and olive oils on fasting and postprandial plasma lipoproteins in humans as part of a National Cholesterol Education Program Step 2 diet Arterioscler. Thromb. 13: 1533–1542

    Article  CAS  Google Scholar 

  • Mensink RP, Katan MB . 1992 Effect of dietary fatty acids on serum lipids and lipoproteins. A meta-analysis of 27 trials Arterioscler. Thromb. 12: 911–919

    Article  CAS  Google Scholar 

  • Mutanen M, Kleemola P, Valsta LM, Mensink RP, Räsänen L . 1992 Lack of effect on blood pressure by polyunsaturated and monounsaturated fat diets Eur. J. Clin. Nutr. 46: 1–6

    CAS  PubMed  Google Scholar 

  • National Public Health Institute, Department of Nutrition. 1998 The 1997 Dietary Survey of Finnish Adults B8, 1988. Printed in Hakapaino Oy, Helsinki: National Public Health Institute

  • Noakes M, Nestel PJ, Clifton PM . 1996 Modifying the fatty acid profile of dairy products through feedlot technology lowers plasma cholesterol of humans consuming the products Am. J. Clin. Nutr. 63: 42–46

    Article  CAS  Google Scholar 

  • Pietinen P, Hartman A, Haapa E, Räsänen L, Haapakoski J, Palmgren J, Albanes D, Virtamo J, Huttunen J . 1988 Reproducibility and validity of dietary assessment instruments, a self-administered food use questionnaire with a portion size picture booklet Am. J. Epidemiol. 128: 655–666

    Article  CAS  Google Scholar 

  • Puska P, Iacono JM, Nissinen A, Vartiainen E, Dougherty R, Pietinen P, Leino U, Uusitalo U, Kuusi U, Kostiainen E, Nikkar T, Seppälä E, Vapaatalo H, Huttunen J . 1985 Dietary fat and blood pressure: an intervention study on the effects of a low-fat diet with two levels of polyunsaturated fat Prev. Med. 14: 573–584

    Article  CAS  Google Scholar 

  • Putnam J, Allshouse J . 1999 Food Consumption, Prices, and Expenditures, 1970–97 Statistical Bulletin 9650 Washington, DC: Food and Rural Economics Division, Economic Research Service, US Department of Agriculture

    Google Scholar 

  • Rastas M, Seppänen R, Knuts LR, Hakala P, Karttila V (eds). 1997 Nutrient Composition of Foods Turku: The Social Insurance Institution, Finland

    Google Scholar 

  • Ritz E, Heuck CC, Boland R . 1980 Phosphate, calcium and lipid metabolism Adv. Exp. Med. Biol. 128: 197–208

    Article  CAS  Google Scholar 

  • Sarkkinen ES, Uusitupa MI, Pietinen P, Aro A, Ahola I, Penttilä I, Kervinen K, Kesäniemi YA . 1994 Long-term effects of three fat-modified diets in hypercholesterolemic subjects Atherosclerosis 105: 9–23

    Article  CAS  Google Scholar 

  • Seppänen-Laakso T, Vanhanen H, Laakso I, Kohtamäki H, Viikari J . 1992 Replacement of butter on bread by rapeseed oil and rapeseed oil-containing margarine: effects on plasma fatty acid composition and serum cholesterol Br. J. Nutr. 68: 639–654

    Article  Google Scholar 

  • Tang JL, Armitage JM, Lancaster T, Silagy CA, Fowler GH, Neil HA . 1998 Systematic review of dietary intervention trials to lower blood total cholesterol in free-living subjects Br. Med. J. 316: 1213–1220

    Article  CAS  Google Scholar 

  • Tholstrup T, Sandstrom B, Hermansen JE, Holmer G . 1998 Effect of modified dairy fat on postprandial and fasting plasma lipids and lipoproteins in healthy young men Lipids 33: 11–21

    Article  CAS  Google Scholar 

  • Valsta LM, Jauhiainen M, Aro A, Katan MB, Mutanen M . 1992 Effects of a monounsaturated rapeseed oil and a polyunsaturated sunflower oil diet on lipoprotein levels in humans Arterioscler. Thromb. 12: 50–57

    Article  CAS  Google Scholar 

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The authors wish to thank medical laboratory technologist Jouni Hodju for excellent laboratory work.

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Correspondence to ES Sarkkinen.

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Karvonen, H., Tapola, N., Uusitupa, M. et al. The effect of vegetable oil-based cheese on serum total and lipoprotein lipids. Eur J Clin Nutr 56, 1094–1101 (2002).

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