Abstract
Objective: To examine the effect of a low phenol olive oil and high phenol olive oil on markers of oxidation and plasma susceptibility to oxidation in normolipaemic smokers.
Design: Randomized single-blind cross-over trial with two intervention periods.
Setting: The Medical School and University Hospital of the University of Crete, Heraklion, Crete, Greece.
Subjects: Twenty-five healthy males and females completed the study.
Interventions: Each intervention was of three weeks duration and intervention periods were separated by a two week washout. Seventy grams of extra virgin olive oil was supplied to each subject per day in the intervention periods. The olive oils supplied differed in their phenol content by 18.6 mg/day. Two fasting venous blood samples were taken at the end of each intervention period.
Results: The markers of antioxidant capacity measured in fasting plasma samples (total plasma resistance to oxidation, concentrations of protein carbonyl as a marker of protein oxidation, malondialdehyde and lipid hydroperoxides as markers of lipid oxidation and the ferric reducing ability of plasma) did not differ significantly between the low and high phenol olive oil diets.
Conclusions: No effect of olive oil phenols on markers of oxidation in smokers was detected. It may be that the natural concentrations of phenols in olive oil are too low to produce an effect in the post-absorptive phase. Possible reasons for period effects and interactions between diet and administration period need attention to aid further cross-over trials of this kind.
Sponsorship: Unilever Research Vlaardingen, The Netherlands.
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References
Armitage P, Berry G . 1987 Statistical Methods in Medical Research 2nd edn; pp 223–225 Oxford: Blackwell Scientific
Aviram M, Eias K . 1993 Dietary olive oil reduces low-density lipoprotein uptake by macrophages and decreases the susceptibility of the lipoprotein to undergo lipid peroxidation Ann. Nutr. Metab. 37: 75–84
Benzie IF, Strain JJ . 1996 The ferric reducing ability of plasma (FRAP) as a measure of ‘antioxidant power’: the FRAP assay Anal. Biochem. 239: 70–76
Bonanome A, Pagnan A, Biffanti S, Opportuno A, Sorgato F, Dorella M, Maiorino M, Ursini F . 1992 Effect of dietary monounsaturated and polyunsaturated fatty acids on the susceptibility of plasma low density lipoproteins to oxidative modification Arterioscler. Thromb. 12: 529–533
Bonanome A, Pagnan A, Caruso D, Toia A, Xamin A, Fedeli E, Berra B, Zamburlini A, Ursini F, Galli G . 2000 Evidence of postprandial absorption of olive oil phenols in humans Nutr. Metab. Cardiovasc. Dis. 10: 111–120
Buss H, Chan TP, Sluis KB, Domigan NM, Winterbourn CC . 1997 Protein carbonyl measurement by a sensitive ELISA method Free Rad. Biol. Med. 23: 361–366
Hills M, Armitage P . 1979 The two-period cross-over clinical trial Br. J. Clin. Pharmac. 8: 7–20
Hulea SA, Olinescu R, Nita S, Crocnan D, Kummerow FA . 1995 Cigarette smoking causes biochemical changes in blood that are suggestive of oxidative stress: a case-control study J. Environ. Pathol. Toxicol. Oncol. 14: (3–4): 173–180
Keys A, Menotti A, Karvonen MJ, Aravanis C, Blackburn H, Buzina R, Djordjevic BS, Dontas AS, Fidanza F, Keys MH, Kromhout D, Nedeljkovic S, Punsar S, Seccareccia F, Toshima H . 1986 The diet and 15-year death rate in the seven countries study Am. J. Epidemiol. 124: 903–915
MacNee W . 2000 Oxidants/antioxidants and COPD Chest 117: (5 Suppl 1): 303S–317S
Mamalakis G, Kafatos A . 2001 Mediterranean diet and longetivity In: The Mediterranean Diet: Constituents and Health Promotion A Matalas, A Zampelas, V Stavrinos & I Wolinsky, pp 205–223 Boca Raton, FL: CRC Press
Montedoro G, Servili M, Baldioli M, Selvaggini R, Miniati E, Macchioni A . 1993 Simple and hydrolyzable compounds in virgin olive oil. III. Spectroscopic characterizations of the secoiridoid derivatives J. Agric. Food Chem. 41: 2228–2234
Nicolaïew N, Lemort N, Adorni L, Berra B, Montorfano G, Rapelli S, Cortesi N, Jacotot B . 1998 Comparison between extra virgin olive oil and oleic acid rich sunflower oil: effects on postprandial lipemia and LDL susceptibility to oxidation Ann. Nutr. Metab. 42: 251–260
Pocock SJ . 1983 Clinical Trials: a Practical Approach pp 114–119 Chichester: John Wiley
Princen HM, van Poppel G, Vogelezang C, Buytenhek R, Kok FJ . 1992 Supplementation with vitamin E but not beta-carotene in vivo protects low density lipoprotein from lipid peroxidation in vitro. Effect of cigarette smoking Arterioscler. Thromb. 12: 554–562
Reaven P, Parthasarathy S, Grasse BJ, Miller E, Almazan F, Mattson FH, Khoo JC, Steinberg D, Witztum JL . 1991 Feasibility of using an oleate-rich diet to reduce the susceptibility of low-density lipoprotein to oxidative modification in humans Am. J. Clin. Nutr. 54: 701–706
Scaccini S, Nardini M, D'Aquino M, Gentili V, Di Felice M, Tomassi G . 1992 Effect of dietary oils on lipid peroxidation and on antioxidant parameters of rat plasma and lipoprotein fractions J. Lipid Res. 33: 627–633
Serra-Majem L, Ribas L, Treserras R, Ngo J, Salleras L . 1995 How could changes in diet explain changes in coronary heart disease mortality in Spain? The Spanish paradox Am. J. Clin. Nutr. 61: (Suppl): 1351S–1359S
Snedecor GW, Cochran WG . 1989 Statistical Methods 8th edn, pp 102–106 Ames, IA: The Iowa State University Press
Visioli F, Galli C, Bornet F, Mattei A, Patelli R, Galli G, Caruso D . 2000 Olive oil phenolics are dose-dependently absorbed in humans FEBS Lett. 468: 159–160
Vissers MN, Zock PL, Leenen R, Roodenburg AJC, van Putte KPAM, Katan MB . 2001a Effect of consumption of phenols from olives and extra virgin olive oil on LDL oxidizability in healthy humans Free Rad. Res. 35: 619–629
Vissers MN, Zock PL, Wiseman SA, Meyboom S, Katan MB . 2001b Effect of phenol-rich extra virgin olive oil on markers of oxidation in healthy volunteers Eur. J. Clin. Nutr. 55: 334–341
Wiseman SA, Mathot JN, de Fouw N, Tijburg LB . 1996 Dietary non-tocopherol antioxidants present in extra virgin olive oil increase the resistance of low density lipoproteins to oxidation in rabbits Atherosclerosis 120: 15–23
Wong SH, Knight JA, Hopfer SM, Zaharia O, Leach-CN J, Sunderman-FW J . 1987 Lipoperoxides in plasma as measured by liquid-chromatographic separation of malondialdehyde-thiobarbituric acid adduct Clin. Chem. 33: 214–220
Zhou JF, Yan XF, Guo FZ, Sun NY, Qian ZJ, Ding DY . 2000 Effects of cigarette smoking and smoking cessation on plasma constituents and enzyme activities related to oxidative stress Biomed. Environ. Sci. 13: 44–55
Acknowledgements
We thank Unilever Research Vlaardingen, The Netherlands for financial support of the project. Many thanks also go to Professor MB Katan for his helpful advice. We are indebted to our research team at the time of the study; in particular thanks go to Dr Christos Hatzis for unfailing organizational and technical support, Giorgia Martimianaki for vitamin analyses, to the dieticians Eva Mouka, Ioanna Apostolaki and Foteini Cheiladaki and to our secretarial administrator, Gianna Kelesi for her assistance in coordination of the study. Finally, many thanks go to the participating staff at Wageningen University and of course to all the volunteers in Heraklion for their time and enthusiastic compliance.
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Moschandreas, J., Vissers, M., Wiseman, S. et al. Extra virgin olive oil phenols and markers of oxidation in Greek smokers: a randomized cross-over study. Eur J Clin Nutr 56, 1024–1029 (2002). https://doi.org/10.1038/sj.ejcn.1601444
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DOI: https://doi.org/10.1038/sj.ejcn.1601444
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