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Energy metabolism in humans at a lowered ambient temperature


Objective: Assessment of the effect of a lowered ambient temperature, ie 16°C (61°F), compared to 22°C (72°F), on energy intake (EI), energy expenditure (EE) and respiratory quotient (RQ) in men.

Design: Randomized within-subject design in which subjects stayed in a respiration chamber three times for 60 h each, once at 22°C, and twice at 16°C, wearing standardized clothing, executing a standardized daily activities protocol, and were fed in energy balance (EBI): no significant difference between EE and EI over 24 h). During the last 24 h at 22°C, and once during the last 24 h at 16°C, they were fed ad libitum.

Subjects: Nine dietary unrestrained male subjects (ages 24±5 y, body mass index (BMI) 22.7±2.1 kg/m2, body weight 76.2±9.4 kg, height 1.83±0.06 m, 18±5% body fat).

Results: At 16°C (EB), EE (total 24 h EE) was increased to 12.9±2.0 MJ/day as compared to 12.2±2.2 MJ/day at 22°C (P<0.01). The increase was due to increases in sleeping metabolic rate (SMR; the lowest EE during three consecutive hours with hardly any movements as indicated by radar): 7.6±0.7 vs 7.2±0.7 MJ/day (P<0.05) and diet-induced thermogenesis (DIT; EE-SMR, when activity induced energy expenditure as indicated by radar=0): 1.7±0.4 vs 1.0±0.4 MJ/day (P<0.01). Physical activity level (PAL; EE/SMR) was 1.63–1.68. At 16°C compared to at 22°C, rectal, proximal and distal skin temperatures had decreased (P<0.01). RQ was not different between the two ambient temperature situations. During ad libitum feeding, subjects overate by 32±12% (at 22°C) and by 34±14% (at 16°C). Under these circumstances, the decrease of rectal temperature at 16°C was attenuated, and inversely related to percentage overeating (r2=0.7; P<0.01).

Conclusion: We conclude that at 16°C, compared to 22°C, energy metabolism was increased, due to increases in SMR and DIT. Overeating under ad libitum circumstances at 16°C attenuated the decrease in rectal core body temperature.

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  • Benzinger TM . 1969 Heat regulation: homeostasis of control temperature in man Physiol. Rev. 49: 671–759

    CAS  Article  Google Scholar 

  • Bergh U, Ekblom B . 1979 Physical performance and peak aerobic power at different body temperatures J. Appl. Physiol. 46: 885–889

    CAS  Article  Google Scholar 

  • Blaza S, Garrow JS . 1983 Thermogenic response to temperature, exercise and food stimuli in lean and obese women, studied by 24 h direct calorimetry Br. J. Nutr. 49: 171–180

    CAS  PubMed  Google Scholar 

  • Bouten CVC, Pannemans DLE, Westerterp KR, Verduin M . 1995 Physical activity assessment during standardized activity in a respiration chamber: movement registration versus energy expenditure Int. J. Obes. Relat. Metab. Disord. 19: 29–35

    Google Scholar 

  • Bouten CVC, van Marken Lichtenbelt WD, Westerterp KR . 1996 Body mass index and daily physical activity in anorexia nervosa Med. Sci. Sports Exerc. 28: 967–973

    CAS  Article  Google Scholar 

  • Brengelmann GL, Savage MV . 1997 Temperature regulation in the neutral zone Ann. N.Y. Acad. Sci. 813: 39–50

    CAS  Article  Google Scholar 

  • Consolazio CF, Matoush L, Le Roy O, Nelson RA . 1963 Environmental temperature and energy expenditures J. Appl. Physiol. 18: 65–68

    CAS  Article  Google Scholar 

  • Dauncey MJ . 1981 Influence of mild cold on 24 h energy expenditure, resting metabolism and diet-induced thermogenesis Br. J. Nutr. 45: 257–267

    CAS  Article  Google Scholar 

  • Du Bois EF . 1936 Basal Metabolism in Health and Disease, 3rd edn Philadelphia, PA: Lea and Febiger

  • Edholm OG, Goldsmith R . 1966 Food intakes and weight changes in climatic extremes Proc. Nutr. Soc. 25: 113–119

    CAS  Article  Google Scholar 

  • Hardy JD . 1934 Radiation of heat from the human body. An instrument for measuring the radiation and the surface temperature of the skin J. Clin. Invest. 13: 593–599

    CAS  Article  Google Scholar 

  • Hardy JD, Du Bois EF . 1934 Basal metabolism, radiation, convection and vaporization at temperatures of 22–35°C J. Nutr. 8: 695–703

    Article  Google Scholar 

  • Hardy JD, Du Bois EF . 1940 Differences between men and women in their response to heat and cold Proc. Natl. Acad. Sci. USA 26: 389–398

    CAS  Article  Google Scholar 

  • Hardy JD, Milhorat AT . 1939 Basal heat loss and production in women at temperatures from 23–36°C Proc. Soc. Exper. Biol. Med. 41: 94

    Article  Google Scholar 

  • ISO 9920 . 1995 Ergonomics of the thermal environment—Estimation of the thermal insulation and evaporative resistance of a clothing ensemble Geneva: IOS

  • Jequier E, Schutz Y . 1983 Long term measurements of energy expenditure in humans using a respiration chamber Am. J. Clin. Nutr. 38: 989–998

    CAS  Article  Google Scholar 

  • Johnson JM . 1979 Reflex control of skin blood flow by skin temperature: role of core temperature J. Appl. Physiol: Respirat. Environ. Exerc. Physiol. 47: 1188–1193

    CAS  Article  Google Scholar 

  • Johnson RE, Kark RM . 1947 Environment and food intake in man Science 105: 378–379

    CAS  Article  Google Scholar 

  • Kobayashi S . 1988 Temperature-sensitive neurons in the hypothalamus; a new hypothesis that they act as thermostats, not as transducers Prog. Neurobiol. 32: 103–135

    Article  Google Scholar 

  • Kraeuchi K, Wirz-Justice A . 1994 Circadian rhythm of heat production, heart rate, and skin and core temperature under unmasking conditions in men Am. J. Physiol. 267: R819–R829

    CAS  Google Scholar 

  • LeBlanc JA . 1957 Effect of environmental temperature on energy expenditure and calorie requirements J. Appl. Physiol. 10: 281–283

    CAS  Article  Google Scholar 

  • Montgomery LD . 1976 Effect of ambient temperature on the thermal profile of the human forearm, hand, and fingers Ann. Biomed. Engng. 4: 209–219

    CAS  Article  Google Scholar 

  • Pannemans DLE, Bouten CVC, Westerterp KR . 1995 24 h energy expenditure during a standardized activity protocol in young and elderly men Eur. J. Clin. Nutr. 49: 49–56

    CAS  PubMed  Google Scholar 

  • Ravussin ES, Lillioja TE, Anderson L, Christin C, Bogardus C . 1986 Determinants of 24-energy expenditure in man J. Clin. Invest. 78: 1568–1578

    CAS  Article  Google Scholar 

  • Refinetti R . 1997 Homeostasis and circadian rhythmicity in the control of body temperature Ann. N.Y. Acad. Sci. 813: 63–70

    CAS  Article  Google Scholar 

  • Rothwell NJ, Stock MJ, Stribling D . 1990 Diet-induced thermogenesis In Thermoregulation, Physiology and Biochemistry Chap International Encyclopedia of Pharmacology and Therapeuties, eds. E Schoenbaum and P Lomax. Sect 131. New York: Pergamon Press

    Google Scholar 

  • Schofflelen PFMA, Westerterp KR, Saris WHM, ten Hoor F . 1997 A dual respiration chamber system with automated calibration J. Appl. Physiol. 83: 2064–2072

    Article  Google Scholar 

  • Siri WE . 1961 Body composition from fluid density: analysis of methods In Techniques for Measuring Body Composition, ed. J Brozek & A Herschel 233–244 Washington, DC: National Academy of Sciences, National Research Council

    Google Scholar 

  • Stunkard AJ, Messick S . 1985 The Three Factor Eating Questionnaire to measure dietary restraint, distribution and hunger Psych. Res. 29: 495–502

    Article  Google Scholar 

  • Vander A, Sherman J, Luciano D . 1998 Regulation of organic metabolism, growth, and energy balance In Human Physiology. The Mechanisms of Body Function, 7th edn pp 625–633 New York: WCB/McGraw-Hill

    Google Scholar 

  • van Marken Lichtenbelt WD, Westerterp-Plantenga MS, Van Hoydonck P . 2001 Effects of ambient temperature on body temperature and energy expenditure in humans Physiol. Behav. 73: 235–242

    Article  Google Scholar 

  • Voorlichtingsbureau voor de Voeding . 1992 De handleiding van de Voedingswijzer. Den Haag

  • Webb P . 1992 Temperatures of skin subcutaneous tissue, muscle and core in resting men in cold, comfortable and hot conditions Eur. J. Appl. Physiol. 64: 471–476

    CAS  Article  Google Scholar 

  • Webb P . 1993 Daily activity and body temperature Eur. J. Appl. Physiol. 66: 174–177

    CAS  Article  Google Scholar 

  • Weir JBDV . 1949 New methods for calculating metabolic rate with special reference to protein metabolism J. Physiol. 109: 1–9

    Article  Google Scholar 

  • Werner J . 1981 Control aspects of human temperature regulation Automatica 17: 351–362

    Article  Google Scholar 

  • Westerterp KR, Meijer GAL, Saris WHM, Soeters PB, Winants Y, ten Hoor F . 1991 Physical activity and sleeping metabolic rate Med. Sci. Sports Exerc. 23: 166–170

    CAS  Article  Google Scholar 

  • Westerterp KR, Wilson SAJ, Rolland V . 1998 Diet induced thermogenesis measured over 24 h in a respiration chamber: effect of diet composition Int. J. Obes. Relat. Metab. Disord. 22: 1–6

    Article  Google Scholar 

  • Westerterp-Plantenga MS, van den Heuvel E, Wouters L, ten Hoor F . 1991 Restrained eating, obesity and cumulative food intake curves during four-course meals Appetite 16: 149–158

    CAS  Article  Google Scholar 

  • Westerterp-Plantenga MS, Rolland V, Wilson SAJ, Westerterp KR . 1999 Satiety related to 24 h diet-induced thermogenesis during high protein/carbohydrate vs high fat diets measured in a respiration chamber Eur. J. Clin. Nutr. 53: 495–502

    CAS  Article  Google Scholar 

  • Westerterp-Plantenga MS, van Marken Lichtenbelt WD, Top S, Cilissen C . Energy metabolism in women during short exposure to the thermoneutral zone. Physiol. Behav. (in press).

  • Wyss CR, Brengelmann GL, Johnson JM . 1974 Control of skin blood flow, sweating and heart rate: role of skin vs core temperature J. Appl. Physiol. 36: 726–733

    CAS  Article  Google Scholar 

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We wish to thank Paul Schoffelen for his assistance with the respiration chamber measurements, Lock Wouters for helping with the body temperature registrations, and Dr Kathleen Melanson for editing the English text.

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Correspondence to MS Westerterp-Plantenga.


Appendix 1 Activity protocol

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Westerterp-Plantenga, M., van Marken Lichtenbelt, W., Strobbe, H. et al. Energy metabolism in humans at a lowered ambient temperature. Eur J Clin Nutr 56, 288–296 (2002).

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  • ambient temperature
  • energy intake
  • energy expenditure
  • body temperature
  • humans

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