Objective: To assess the utility of serum phospholipid fatty acid (FA) levels as a biochemical indicator of habitual dietary fatty acid intake in Japanese, whose diet is characterized by low fat intake and high intake of n-3 polyunsaturated fatty acids (PUFA) of marine origin.
Subjects and methods: Eighty-seven male volunteers from four public health center districts that were part of the Japan Public Health Center based Prospective Study (JPHC Study) cohort I, were included in this study. Habitual intake of fatty acid was obtained by 7 day weighed dietary records four times (in one area only twice) in 1994–1995. Blood was collected twice, in February and August of the same year, and the composition of FA in serum phospholipid was analyzed by gas chromatography. The correlation coefficient between serum phospholipid FA levels and fatty acid intake was calculated.
Results: High correlations were observed for eicosapentaenoic acid (EPA), docosapentaenoic acid (DPA) and docosahexaenoic acid (DHA), which are marine origin n-3 PUFA (r=0.75, 0.49, 0.50, respectively). No significant correlation was observed for saturated fatty acid (SFA), although the monounsaturated fatty acid (MUFA), palmitoleic acid and oleic acid intake were moderately correlated (r=0.22, 0.35, respectively). The correlations for EPA, DPA and DHA were similar in both samples collected in February and August.
Conclusions: These data suggest that in populations with a high and stable over time intake of n-3 PUFA of marine origin, a single measurement of serum phospholipids reflects the ranking of habitual intake of marine origin n-3 PUFA.
European Journal of Clinical Nutrition (2001) 55, 643–650
Andersen LF, Solvoll K & Drevon CA (1996) Very-long-chain n-3 fatty acids as biomarkers for intake of fish and n-3 fatty acid concentrates Am. J. Clin. Nutr. 64 305–311
Andersen LF, Solvoll K, Johansson LR, Salminen I, Aro A & Drevon CA (1999) Evaluation of a food frequency questionnaire with weighed records, fatty acids, and alpha-tocopherol in adipose tissue and serum Am. J. Epidemiol. 150 75–87
Byers T & Gieseker K (1997) Issue in the design and interpretation of studies of fatty acids and cancer in humans Am. J. Clin. Nutr. 66 (Suppl), 1541–1547
Han EK & Paik HY (1995) Relationship between plasma, erythrocyte membrane, and dietary intake levels of ω-3 fatty acids in young Korean females: effect of diet survey for two months Korean J. Nutr. 28 995–1003
Hjartaker A, Lund E & Bjerve KS (1997) Serum phospholipid fatty acid composition and habitual intake of marine foods registered by a semi-quantitative food frequency questionnaire Eur. J. Clin. Nutr. 51 736–742
Hunter DJ, Rimm EB, Sacks FM, Stampfer MJ, Colditz GA, Litin LB & Willett WCI (1992) Comparison of measures of fatty acid intake by subcutaneous fat aspirate, food frequency questionnaire, and diet records in a free-living population of US men Am. J. Epidemiol. 135 418–427
Iso H, Sato S, Folsom AR, Shimamoto T, Terao A, Munger RG, Kitamura A, Konishi M, lida M & Komachi Y (1989) Serum fatty acids and fish intake in rural Japanese, urban Japanese, Japanese American and Caucasian American men Int. J. Epidemiol. 18 374–381
Kobayashi M, Sasaki S, Hamada GS & Tsugane S (1999) Serum n-3 fatty acids, fish consumption and cancer mortality in six Japanese populations in Japan and Brazil Jpn J. Cancer Res. 90 914–921
Kohlmeier L (1997) Biomarkers of fatty acid exposure and breast cancer risk Am. J. Clin. Nutr. 66 (Suppl), 1548–1556
Leaf DA, Connor WE, Barstad L & Sexton G (1995) Incorporation of dietary n-3 fatty acids into the fatty acids of human adipose tissue and plasma lipid classes Am. J. Clin. Nutr. 62 68–73
Ma J, Folsom AR, Shahar E & Eckfeldt JH (1995) Plasma fatty acid composition as an indicator of habitual dietary fat intake in middle-aged adults: the Atherosclerosis Risk in Communities (ARIC) Study Investigators Am. J. Clin. Nutr. 62 564–571
Ministry of Health and Welfare (1997) Kokumin Eiyou no Genjyou (Results of National Nutrition Survey, 1995) pp 74, 109, 11 3 Tokyo: Daiichi Shuppan (in Japanese).
Nerson GJ (1998) Dietary fat, trans fatty acids, and risk of coronary heart disease Nutr. Rev. 56 250–252
Nikkari T, Luukkainen P, Pietinen P & Puska P (1995) Fatty acid composition of serum lipid fractions in relation to gender and quality of dietary fat Ann. Intern. Med. 27 491–498
Okita M, Yoshida S, Yamamoto J, Suzuki K, Kaneyuki T, Kubota M & Sasagawa T (1995) N-3 and n-6 fatty acid intake and serum phospholipid fatty acid composition in middle-aged women living in rural and urban areas in Okayama Prefecture J. Nutr. Sci. Vitaminol. 41 313–323
Riboli E, Ronnholm H & Saracci R (1987) Biological markers of diet Cancer Surv. 6 685–718
Rose DP (1997a) Dietary fatty acids and cancer Am. J. Clin. Nutr. 66 (Suppl), 998–1003
Rose DP (1997b) Dietary fatty acids and prevention of hormone-responsive cancer Proc. Soc. Exp. Biol. Med. 216 224–233
SAS Institute Inc. (1989) SAS/STAT User's Guide Version 6.4, Vol 2. Cary, NC: SAS Institute Inc.
Sasaki S, Kobayashi M & Tsugane S (1999) Development of substituted fatty acid food composition table for the use in nutritional epidemiologic studies for Japanese populations: its methodological backgrounds and the evaluation J. Epidemiol. 9 190–207
Sato S (1990) Dietary and serum fatty acid compositions among six Japanese populations Nippon Koshu Eisei Zasshi 37 498–508 (in Japanese).
Science and Technology Agency (1982) Standard Tables of Food Composition in Japan: The fourth revised edition. Tokyo: Printing Bureau, Ministry of Finance (in Japanese).
Science and Technology Agency (1990) Fatty Acids, Cholesterol, Vitamin E Composition Table of Japanese Foods. Tokyo: Ishiyaku Shuppan Publishers (in Japanese).
Seidelin KN (1995) Fatty acid composition of adipose tissue in humans: Implications for the dietary fat-serum cholesterol-CHD issue Prog. Lipid Res. 34 199–217
Tjonneland A, Overvad K, Thorling E & Ewertz M (1993) Adipose tissue fatty acids as biomarkers of dietary exposure in Danish men and women Am. J. Clin. Nutr. 57 629–633
Tsugane S, Fahey MT, Sasaki S & Baba S (1999) Alcohol consumption and all-cause and cancer mortality among middle-aged Japanese men: seven-year follow-up of the JPHC study Cohort I. Japan Public Health Center Am. J. Epidemiol. 150 1201–1207
von Schacky C (2000) n-3 fatty acids and the prevention of coronary atherosclerosis Am. J. Clin. Nutr. 71 (Suppl), 224s–227s
Willett W (1998) Nutritional Epidemiology, p 22 3 New York: Oxford University Press
The authors express their appreciation to the local staff in each study area, especially to the following dietitians for their painstaking efforts in conducting the dietary survey: Ms Yuko Hatakeyama, Mayumi Morimoto, Masako Makino, Manami Nishizawa, Misako Tawada, Noriko Kamiunten and Satoko Takamori. We also thank Drs Youji litoi, Yasuhiko Iwase, and Tosei Takahashi for data management and Dr Seiichiro Yamamoto for statistical advice. This work was supported by grants-in-aid for cancer research and for the Second Term Comprehensive Ten-Year Strategy for Cancer Control from the Ministry of Health and Welfare of Japan and for grants-in aid for scientific research from the Ministry of Education, Science, Sports and Culture of Japan. MK was awarded a Research Resident Fellowship from the Foundation for Promotion of Cancer Research in Japan.
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Fish and Meat Intake, Serum Eicosapentaenoic Acid and Docosahexaenoic Acid Levels, and Mortality in Community-Dwelling Japanese Older Persons
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