Abstract
Mehl announced in 19451 that by the use of ethylene glycol an alkaline copper reagent could be prepared in which copper, besides not being precipitated, could give the biuret reaction. At the high proportion in which glycol enters into Mehl's reagent, the presence of reducing impurities made it necessary to prepare the reagent in two stages, heating in order to speed up the reaction, and filtering in order to remove the precipitated copper. Nevertheless, the presence of the colour of the alkaline copper reagent causes the reaction to depart considerably from Beer's law; Mehl deduces from a spectrophotometric analysis that, starting from the 750 mµ and 545 mµ absorptions, the quantity of proteins present can be approximately calculated.
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References
Mehl, J., J. Biol. Chem., 157, 173 (1945).
Sols, A., R. esp. Fisiol., 1, 355 (1945).
Sols, A., An. Fis. Quim., 42, 855 (1946).
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SOLS, A. An Improved Biuret Reaction of Proteins and the Two-Standard Colorimetry. Nature 160, 89 (1947). https://doi.org/10.1038/160089a0
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DOI: https://doi.org/10.1038/160089a0
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