Letter | Published:

Tetrazolium Salt as a Seed Germination Indicator

Nature volume 159, page 748 (31 May 1947) | Download Citation



A TOPOGRAPHICAL chemical test for germination was devised by Lakon1 using sodium biselenite, which, on account of its toxicity, he rejected in favour of the tetrazolium salts2. Later, he evolved a technique for maize3 with 2,3,5-triphenyl tetrazolium chloride, which was used in Germany during the War4. As information about other cereals was inadequate, a preliminary assessment of this compound as a germination indicator has now been made.

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  1. 1.

    , Ber. deut. bot. Ges., 57, 191 (1939).

  2. 2.

    , Ber. deut. bot. Ges., 60, 299 (1942).

  3. 3.

    , Ber. deut. bot. Ges., 60, 434 (1942).

  4. 4.

    C.I.O.S. Report, Item 24, No. XXVI–73.

  5. 5.

    and , Ber. deut. chem. Ges., 74B, 941.

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  1. Research Laboratories, May and Baker, Ltd., Dagenham.

    • H. J. COTTRELL


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