Abstract
SCHIFF1 observed that cultures and culture filtrates of certain strains of Clostridium welchii possess the power to inactivate the blood-group A -substance contained in jpeptone and human saliva. The decompositiony -as considered specific for A -substance. We have examined a number of crude Cl. welchii filtrates fi r their capacity to destroy the specific blood-gr up substances, but have found them disappointingly weak. Through the kindness of Dr. W. EC van Heyningen, who has supplied a number of welchii (Type A) culture filtrate preparations partially purified with reference to collagenase (x-toxin)2, it has been possible to study the action of the enzymes contained in these preparations on the blood-group substances. The filtrates contained collagenase and hyaluronidase3, and in most specimens a- and 0-toxins were also present. The substrates used were preparations of -substance obtained from hog gastric mucin4 and A-, B- and O-substances which had been isolated from human pseudomucinous ovarian cyst fluids5. The human A- and 5-substances showed no significant O specificity. The A -substance isolated from hog mucin, although electrophoretically homogeneous at pH. 4.0 and 8.0, nevertheless is composed of two mucoids, one of which possesses A specificity and the other O specificity. The mixed material, which is usually referred to as hog mucin 4-substance, cannot be separated readily into its sero-logically specific A and O components by any of the simple chemical or physical techniques employed so far, but full details of this aspect of the dual specificity of hog mucin '.4-substance' and of preparations of A- and B-substances isolated from the saliva and gastric juice of persons belonging to groups A, B and AB will be discussed elsewhere.
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References
Schiff, F., Klin. Wochschr., 14, 750 (1935). J. Infect. Dis., 65, 127 (1939).
Maschmann, Biochem. Z., 295, 391 (1938). Oakley, C. L., Warrack, H. G., and van Heyningen, W. E., J. Path, and Bact., 58, 229 (1946).
Robertson, W. V. B., Ropes, M. W., and Bauer, W., Amer. J. Physiol., 126, 609 (1939). Meyer, K., Hobby, G. L., Chaffee, E., and Dawson, M. H., J. Exp. Med., 71, 137 (1940).
Meyer, K., Smyth, E., and Palmer, J., J. Biol. Chem., 119, 73 (1937). Landsteiner, K., and Harte, R. A., J. Exp. Med., 71, 551 (1940). Morgan, W. T. J., and King, H. K., Biochem. J., 37, 640 (1943). Morgan, W. T. J., Brit. J. Exp. Path., 24, 41 (1943).
Morgan, W. T. J., and van Heyningen, R., Brit. J. Exp. Path., 25, 5 (1944). Morgan, W. T. J., and Watkins, W. M., Brit. J. Exp. Path., 25, 221 (1944). King, H. K., and Morgan, W. T. J., Biochem. J., 38, X (Proc.) (1944). Morgan, W. T. J., and Waddell, M. B. R., Brit. J. Exp. Path., 26, 387 (1945).
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MORGAN, W. Enzymic Decomposition of A, B and O Specific Blood-Group Substances. Nature 158, 759–760 (1946). https://doi.org/10.1038/158759a0
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DOI: https://doi.org/10.1038/158759a0
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