Article | Published:

A Microchromatographic Method for the Detection and Approximate Determination of the Different Penicillins in a Mixture

Nature volume 158, pages 675676 (09 November 1946) | Download Citation

Subjects

Abstract

MARTIN and co-workers1 extended their technique of partition chromatography of the amino-acids to the micro-scale by using filter paper instead of silica gel as the support for the stationary phase (water). We have found that the modified partition chromatogram for penicillin using buffer as the stationary phase2 can also be adapted to the micro-scale by the same change in support. Colour reactions are not readily applicable to locate the positions of the invisible developed zones, so that a microbiological procedure has been used both to identify and to determine approximately the types and amounts of penicillins resolved on the buffered paper strip. The procedure is as follows.

Access optionsAccess options

Rent or Buy article

Get time limited or full article access on ReadCube.

from$8.99

All prices are NET prices.

References

  1. 1.

    , , and , Biochem. J., 38, 224 (1944).

  2. 2.

    , and , Brit. Pat. 569844.

  3. 3.

    , , and , J. Bact., 49, 411 (1945).

Download references

Author information

Affiliations

  1. Research Laboratories, Imperial Chemical Industries, Ltd., Blackley, Manchester 9

    • R. R. GOODALL
    •  & A. A. LEVI

Authors

  1. Search for R. R. GOODALL in:

  2. Search for A. A. LEVI in:

About this article

Publication history

Published

DOI

https://doi.org/10.1038/158675a0

Further reading

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.